研究者詳細 - 宮崎　淳

論文 - 宮崎　淳

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多波長励起光を備えた光熱変換顕微鏡による水中でのチオリンゴ酸修飾銀ナノ粒子の凝集挙動の定量評価

中原佳夫, 宮崎淳, 廣野碧唯, 家永隆史, 矢嶋摂子

分析化学 74 23 - 28 2025年01月 [査読有り]

Photothermal imaging of cellular responses to glucose deprivation

J. Miyazaki, R. Wagatsuma, K. Okamoto (担当区分：筆頭著者,　責任著者 )

RSC Chemical Biology 6 571 - 582 2025年01月 [査読有り]

DOI

Polydopamine-Coated Solid Silica Nanoparticles Encapsulating IR-783 Dyes: Synthesis and NIR Fluorescent Cell Imaging

Yoshio Nakahara, Haruki Nakabayashi, Jun Miyazaki, Mitsuru Watanabe, Toshiyuki Tamai, Setsuko Yajima

ACS Omega 9 19932 - 19939 2024年04月 [査読有り]

Discrimination of individual semiconducting and metallic single-walled carbon nanotubes by multi-wavelength photothermal microscopy

Jun Miyazaki, Honoka Maeda (担当区分：筆頭著者,　責任著者 )

Jpn. J. Appl. Phys 62 102003 2023年10月 [査読有り]

DOI

Quantitative Discrimination of Silver and Gold Nanoparticles Immobilized in Transparent Plastic Film by Photothermal Microscopy with Multiwavelength Excitation

Yoshio Nakahara, Jun Miyazaki, Aoi Hirono, Takashi Ienaga, Setsuko Yajima

Chemistry Letters 52 ( 2 ) 113 - 115 2023年02月 [査読有り]

DOI

Multiwavelength Photothermal Imaging of Individual Single-Walled Carbon Nanotubes Suspended in a Solvent

Jun Miyazaki, Yuya Ishikawa, Ryosuke Kondo (担当区分：筆頭著者,　責任著者 )

The Journal of Physical Chemistry A ( American Chemical Society (ACS) ) 126 ( 32 ) 5483 - 5491 2022年08月 [査読有り]

DOI

Synthesis of Silica Nanoparticles with Physical Encapsulation of Near-Infrared Fluorescent Dyes and Their Tannic Acid Coating

Yoshio Nakahara, Yukiho Nakajima, Soichiro Okada, Jun Miyazaki, Setsuko Yajima

ACS Omega 6 ( 27 ) 17651 - 17659 2021年06月 [査読有り]

Label-free dynamic imaging of mitochondria and lysosomes within living cells via simultaneous dual-pump photothermal microscopy

J. Miyazaki and Y. Toumon (担当区分：筆頭著者,　責任著者 )

Biomedical Optics Express 10 5852 - 5861 2019年 [査読有り]

Experimental evaluation of temperature increase and associated detection sensitivity in shot noise-limited photothermal microscopy

J. Miyazaki and Y. Toumon (担当区分：筆頭著者,　責任著者 )

Optics Communications 430 170 - 175 2019年 [査読有り]

光熱変換顕微鏡によるバイオイメージング

宮崎淳,小林孝嘉 (担当区分：筆頭著者,　責任著者 )

分光研究 67 ( 2 ) 73 - 85 2018年 [査読有り]

Resolution enhancement of pump–probe microscope with an inverse-annular filter

T. Kobayashi, K. Kawasumi, J. Miyazaki and K. Nakata

Optical Review 25 271 - 294 2018年 [査読有り]

Photothermal imaging of skeletal muscle mitochondria

T. Tomimatsu, J. Miyazaki, Y. Kano, T. Kobayashi

BIOMEDICAL OPTICS EXPRESS ( OPTICAL SOC AMER ) 8 ( 6 ) 2965 - 2975 2017年06月 [査読有り]

The morphology and topology of mitochondria provide useful information about the physiological function of skeletal muscle. Previous studies of skeletal muscle mitochondria are based on observation with transmission, scanning electron microscopy or fluorescence microscopy. In contrast, photothermal (PT) microscopy has advantages over the above commonly used microscopic techniques because of no requirement for complex sample preparation by fixation or fluorescent-dye staining. Here, we employed the PT technique using a simple diode laser to visualize skeletal muscle mitochondria in unstained and stained tissues. The fine mitochondrial network structures in muscle fibers could be imaged with the PT imaging system, even in unstained tissues. PT imaging of tissues stained with toluidine blue revealed the structures of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria and the swelling behavior of mitochondria in damaged muscle fibers with sufficient image quality. PT image analyses based on fast Fourier transform (FFT) and Grey-level co-occurrence matrix (GLCM) were performed to derive the characteristic size of mitochondria and to discriminate the image patterns of normal and damaged fibers. (C) 2017 Optical Society of America

DOI

Improvement of signal-to-noise ratio in photothermal microscopy by optimizing detection aperture

J. Miyazaki (担当区分：筆頭著者,　責任著者 )

OPTICS COMMUNICATIONS ( ELSEVIER SCIENCE BV ) 390 99 - 104 2017年05月 [査読有り]

We present a method for improving the signal-to-noise ratio in photothermal microscopy by optimizing the detection aperture in the spatially segmented balanced detection scheme. Experimental studies show a 1.44-fold improvement in the signal-to-noise ratio compared with the previous scheme. Consequently, the ratio is 9.6 times larger than that in conventional detection. The proposed method is implemented in a laser scanning photothermal microscope using low-cost compact laser diodes as the light source.

DOI

Photothermal microscopy for high sensitivity and high resolution absorption contrast imaging of biological tissues

J. Miyazaki and T. Kobayashi (担当区分：筆頭著者 )

Photonics 4 32 2017年 [査読有り]

Resolution enhancement of pump-probe microscopy with an inverse-annular spatial filter

T. Kobayashi, K. Kawasumi, J. Miyazaki, K. Nakata

AIP ADVANCES ( AMER INST PHYSICS ) 6 ( 12 ) 2016年12月 [査読有り]

We have introduced a pupil filter, an inverse-annular pupil filter in a pump-probe photothermal microscope, which provides resolution enhancement in three dimensions. The resolution is probed to be improved in lateral and axial resolution by imaging experiment using 20 nm gold nanoparticles. The improvement in X (perpendicular to the common pump and probe polarization direction), Y (parallel to the polarization direction), and Z (axial direction) are by 15 +/- 6, 8 +/- 8, and 21 +/- 2 % from the resolution without a pupil filter. The resolution enhancement is even better than the calculation using vector field, which predicts the corresponding enhancement of 11, 8, and 6 %. The discussion is made to explain the unexpected results. We also demonstrate the photothermal imaging of thick biological samples (cells from rabbit intestine and kidney) stained with hematoxylin and eosin dye with the inverse-annular filter. (C) 2016 Author(s).

DOI

Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy

Jun Miyazaki, Tadatsune Iida, Shinji Tanaka, Akiko Hayashi-Takagi, Haruo Kasai, Shigeo Okabe, Takayoshi Kobayashi (担当区分：筆頭著者,　責任著者 )

BIOMEDICAL OPTICS EXPRESS ( OPTICAL SOC AMER ) 7 ( 5 ) 1702 - 1710 2016年05月 [査読有り]

A fast, high-sensitivity photothermal microscope was developed by implementing a spatially segmented balanced detection scheme into a laser scanning microscope. We confirmed a 4.9 times improvement in signal-to-noise ratio in the spatially segmented balanced detection compared with that of conventional detection. The system demonstrated simultaneous bi-modal photothermal and confocal fluorescence imaging of transgenic mouse brain tissue with a pixel dwell time of 20 mu s. The fluorescence image visualized neurons expressing yellow fluorescence proteins, while the photothermal signal detected endogenous chromophores in the mouse brain, allowing 3D visualization of the distribution of various features such as blood cells and fine structures probably due to lipids. This imaging modality was constructed using compact and cost-effective laser diodes, and will thus be widely useful in the life and medical sciences. (C) 2016 Optical Society of America

DOI

Reduction of distortion in photothermal microscopy and its application to the high-resolution three-dimensional imaging of nonfluorescent tissues

Jun Miyazaki, Hiromichi Tsurui, Takayoshi Kobayashi (担当区分：筆頭著者 )

BIOMEDICAL OPTICS EXPRESS ( OPTICAL SOC AMER ) 6 ( 9 ) 3217 - 3224 2015年09月 [査読有り]

A scheme for reducing image distortion in photothermal microscopy is presented. In photothermal microscopy, the signal shape exhibits twin peaks corresponding to the focusing or defocusing of the probe beam when a sample is scanned in the axial direction. This causes a distortion when imaging a structured sample in the axial plane. Here, we demonstrate that image distortion caused by the twin peaks is effectively suppressed by providing a small offset between two the focal planes of the pump and the probe beams. Experimental results demonstrate improvement in resolution, especially in the axial direction, over conventional optical microscopy-even with the focal offset. When a dry objective lens with a numerical aperture of 0.95 is used, the full width at half the maximum of the axial point spread function is 0.6 mu m, which is 50% (62%) smaller than the focal spot sizes of the pump (probe) beam. Herein, we present high-resolution three-dimensional imaging of thick biological tissues based on the present scheme. (C) 2015 Optical Society of America

DOI

Sub-diffraction-limit imaging using mode multiplexing

Nan Wang, Jun Miyazaki, Jinping He, Keisuke Seto, Takayoshi Kobayashi

OPTICS COMMUNICATIONS ( ELSEVIER SCIENCE BV ) 343 28 - 33 2015年05月 [査読有り]

Pixel-by-pixel processed fluorescence difference microscopy is experimentally demonstrated by multiplexing excitation laser beams with Gaussian and donut spot shapes and then demultiplexing the fluorescent signals using lock-in amplifiers. With this scheme, a fixed sample of fluorescent spheres and a slice of mouse brain tissue are imaged with resolutions that exceed the diffraction limit. Compared to previously reported subtraction imaging techniques, this pixel-by-pixel scan can be applied to improve the resolution of a moving sample without introducing subtraction errors. The synchronized signal detection feature makes this method extendible to various applications. (C) 2014 Elsevier B.V. All rights reserved.

DOI

Label-free imaging of melanoma with nonlinear photothermal microscopy

Jinping He, Jun Miyazaki, Nan Wang, Hiromichi Tsurui, Takayoshi Kobayashi

OPTICS LETTERS ( OPTICAL SOC AMER ) 40 ( 7 ) 1141 - 1144 2015年04月 [査読有り]

Nonlinear photothermal microscopy, in which the intensity of the pump heating beam is modulated at f and the photothermal signal is extracted from the probe beam with a lock-in amplifier referred to 2f, is applied to the imaging of mouse melanoma without any staining. The pump and probe pulses, with central wavelengths of 488 and 632 nm, and a pulse duration of similar to 100 ps, are filtered from a compact commercial supercontinuum fiber laser source. An auto-balanced detector is applied to accumulate the signal and remove the laser noise of the probe. The spatial resolution of the nonlinear photothermal imaging is enhanced by similar to 18% in both theoretical calculations and experiments, compared with a linear photothermal mechanism, and the resolution enhancement is theoretically similar to 42% compared with conventional optical microscopy. This imaging technique shows possibilities for the clinical evaluation of melanoma with a high contrast and spatial resolution. (C) 2015 Optical Society of America

DOI

Biological imaging with nonlinear photothermal microscopy using a compact supercontinuum fiber laser source

Jinping He, Jun Miyazaki, Nan Wang, Hiromichi Tsurui, Takayoshi Kobayashi

OPTICS EXPRESS ( OPTICAL SOC AMER ) 23 ( 8 ) 9762 - 9771 2015年04月 [査読有り]

Nonlinear photothermal microscopy is applied in the imaging of biological tissues stained with chlorophyll and hematoxylin. Experimental results show that this type of organic molecules, which absorb light but transform dominant part of the absorbed energy into heat, may be ideal probes for photothermal imaging without photochemical toxicity. Picosecond pump and probe pulses, with central wavelengths of 488 and 632 nm, respectively, are spectrally filtered from a compact supercontinuum fiber laser source. Based on the light source, a compact and sensitive super-resolution imaging system is constructed. Further more, the imaging system is much less affected by thermal blurring than photothermal microscopes with continuous-wave light sources. The spatial resolution of nonlinear photothermal microscopy is similar to 188 nm. It is similar to 23% higher than commonly utilized linear photothermal microscopy experimentally and similar to 43% than conventional optical microscopy theoretically. The nonlinear photothermal imaging technology can be used in the evaluation of biological tissues with high-resolution and contrast. (C) 2015 Optical Society of America

DOI

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