Updated on 2024/12/03

写真a

 
SAKAMOTO Takashi
 
Name of department
Faculty of Systems Engineering, Chemistry
Job title
Associate Professor
Mail Address
E-mail address
Homepage
External link

Education

  • 2001
    -
    2005

    Kyoto Institute of Technology   Graduate School of Science and Technology   工芸科学研究科博士後期課程  

Degree

  • 博士(学術)

Academic & Professional Experience

  • 2016.10
    -
    Now

    Wakayama University   Graduate School of Systems Engineering   准教授

  • 2009.06
    -
    2016.09

    Japan Advanced Institute of Science and Technology   マテリアルサイエンス研究科   助教

  • 2007.04
    -
    2009.05

    京都大学大学院   工学研究科   博士研究員

  • 2005.04
    -
    2007.03

    Kyoto Institute of Technology   Graduate School of Science and Technology   博士研究員

Research Areas

  • Nanotechnology/Materials / Chemical biology

  • Life sciences / Bioorganic chemistry

Classes (including Experimental Classes, Seminars, Graduation Thesis Guidance, Graduation Research, and Topical Research)

  • 2023   Scientific and Technical English B   Specialized Subjects

  • 2023   Graduation Research   Specialized Subjects

  • 2023   Experiments in Applied Chemistry   Specialized Subjects

  • 2023   Advanced Lectures in Chemistry   Specialized Subjects

  • 2023   Experiments in Chemistry Ⅲ   Specialized Subjects

  • 2023   Practices in Chemistry   Specialized Subjects

  • 2023   Experiments in Chemistry IV   Specialized Subjects

  • 2023   Practices in Chemoinformatics   Specialized Subjects

  • 2023   Basic Life Science   Specialized Subjects

  • 2023   Life Science   Specialized Subjects

  • 2022   Graduation Research   Specialized Subjects

  • 2022   Graduation Research   Specialized Subjects

  • 2022   Experiments in Material Science andChemistry C   Specialized Subjects

  • 2022   Seminar in Material Science and Chemistry ⅡA   Specialized Subjects

  • 2022   Seminar in Material Science and Chemistry ⅠA   Specialized Subjects

  • 2022   Life Science   Specialized Subjects

  • 2022   Practices in Chemoinformatics   Specialized Subjects

  • 2022   Basic Life Science   Specialized Subjects

  • 2022   Scientific and Technical English B   Specialized Subjects

  • 2022   Advanced Lectures in Chemistry   Specialized Subjects

  • 2022   Experiments in Chemistry IV   Specialized Subjects

  • 2022   Experiments in Chemistry Ⅲ   Specialized Subjects

  • 2022   Practices in Chemistry   Specialized Subjects

  • 2022   Experiments in Applied Chemistry   Specialized Subjects

  • 2022   Nanotechnologies Ⅱ   Specialized Subjects

  • 2022   Introductory Seminar in Systems Engineering   Specialized Subjects

  • 2021   Graduation Research   Specialized Subjects

  • 2021   Experiments in Material Science andChemistry C   Specialized Subjects

  • 2021   Seminar in Material Science and Chemistry ⅡA   Specialized Subjects

  • 2021   Seminar in Material Science and Chemistry ⅠA   Specialized Subjects

  • 2021   Life Science   Specialized Subjects

  • 2021   Experiments in Chemistry Ⅲ   Specialized Subjects

  • 2021   Advanced Lectures in Chemistry   Specialized Subjects

  • 2021   Practices in Chemistry   Specialized Subjects

  • 2021   Experiments in Applied Chemistry   Specialized Subjects

  • 2021   Graduation Research   Specialized Subjects

  • 2021   Graduation Research   Specialized Subjects

  • 2021   Practices in Chemoinformatics   Specialized Subjects

  • 2021   Basic Life Science   Specialized Subjects

  • 2021   Scientific and Technical English B   Specialized Subjects

  • 2021   Experiments in Chemistry IV   Specialized Subjects

  • 2021   Nanotechnologies Ⅱ   Specialized Subjects

  • 2020   Graduation Research   Specialized Subjects

  • 2020   Graduation Research   Specialized Subjects

  • 2020   Introductory Seminar in Systems Engineering   Specialized Subjects

  • 2020   Experiments in Chemistry IV   Specialized Subjects

  • 2020   Practices in Chemoinformatics   Specialized Subjects

  • 2020   Experiments in Chemistry Ⅲ   Specialized Subjects

  • 2020   Life Science   Specialized Subjects

  • 2020   Basic Life Science   Specialized Subjects

  • 2020   Scientific and Technical English B   Specialized Subjects

  • 2020   Experiments in Applied Chemistry   Specialized Subjects

  • 2020   Advanced Lectures in Chemistry   Specialized Subjects

  • 2020   Seminar in Material Science and Chemistry ⅡA   Specialized Subjects

  • 2020   Seminar in Material Science and Chemistry ⅠA   Specialized Subjects

  • 2020   Practices in Chemistry   Specialized Subjects

  • 2020   Nanotechnologies Ⅱ   Specialized Subjects

  • 2020   Experiments in Material Science andChemistry C   Specialized Subjects

  • 2019   Practices in Chemoinformatics   Specialized Subjects

  • 2019   Scientific and Technical English B   Specialized Subjects

  • 2019   Experiments in Material Science and Chemistry   Specialized Subjects

  • 2019   Experiments in Applied Chemistry   Specialized Subjects

  • 2019   Advanced Lectures in Chemistry   Specialized Subjects

  • 2019   Seminar in Material Science and Chemistry ⅡA   Specialized Subjects

  • 2019   Seminar in Material Science and Chemistry ⅠA   Specialized Subjects

  • 2019   Practices in Chemistry   Specialized Subjects

  • 2019   Nanotechnologies Ⅱ   Specialized Subjects

  • 2019   Nanotechnologies Ⅰ   Specialized Subjects

  • 2019   Experiments in Material Science andChemistry B   Specialized Subjects

  • 2019   Experiments in Material Science andChemistry C   Specialized Subjects

  • 2019   Experiments in Chemistry Ⅲ   Specialized Subjects

  • 2019   Life Science   Specialized Subjects

  • 2019   Basic Life Science   Specialized Subjects

  • 2018   Life Science   Specialized Subjects

  • 2018   Basic Life Science   Specialized Subjects

  • 2018   Scientific and Technical English B   Specialized Subjects

  • 2018   Experiments in Material Science and Chemistry   Specialized Subjects

  • 2018   Experiments in Applied Chemistry   Specialized Subjects

  • 2018   Advanced Lectures in Chemistry   Specialized Subjects

  • 2018   Seminar in Material Science and Chemistry ⅡA   Specialized Subjects

  • 2018   Seminar in Material Science and Chemistry ⅠA   Specialized Subjects

  • 2018   Practices in Chemistry   Specialized Subjects

  • 2018   Nanotechnologies Ⅱ   Specialized Subjects

  • 2018   Experiments in Material Science andChemistry C   Specialized Subjects

  • 2017   Advanced Lectures in Chemistry   Specialized Subjects

  • 2017   Experiments in Applied Chemistry   Specialized Subjects

  • 2017   Life Science   Specialized Subjects

  • 2017   Seminar in Material Science and Chemistry ⅡA   Specialized Subjects

  • 2017   Practices in Chemistry   Specialized Subjects

  • 2017   Nanotechnologies Ⅰ   Specialized Subjects

  • 2017   Experiments in Material Science andChemistry C   Specialized Subjects

  • 2017   Experiments in Material Science andChemistry B   Specialized Subjects

  • 2017   Basic Life Science   Specialized Subjects

  • 2017   Experiments in Material Science and Chemistry   Specialized Subjects

  • 2017   Introductory Seminar in Systems Engineering   Specialized Subjects

  • 2017   Seminar in Material Science and Chemistry ⅠA   Specialized Subjects

  • 2017   Nanotechnologies Ⅱ   Specialized Subjects

▼display all

Classes

  • 2023   Systems Engineering SeminarⅠA   Master's Course

  • 2023   Systems Engineering SeminarⅠB   Master's Course

  • 2023   Systems Engineering SeminarⅡA   Master's Course

  • 2023   Systems Engineering SeminarⅡB   Master's Course

  • 2023   Introduction to Chemical Biology   Master's Course

  • 2023   Systems Engineering Project SeminarⅠA   Master's Course

  • 2023   Systems Engineering Project SeminarⅠB   Master's Course

  • 2023   Systems Engineering Project SeminarⅡA   Master's Course

  • 2023   Systems Engineering Project SeminarⅡB   Master's Course

  • 2023   Systems Engineering Advanced Seminar Ⅰ   Doctoral Course

  • 2023   Systems Engineering Advanced Seminar Ⅰ   Doctoral Course

  • 2023   Systems Engineering Advanced Seminar Ⅱ   Doctoral Course

  • 2023   Systems Engineering Advanced Seminar Ⅱ   Doctoral Course

  • 2023   Systems Engineering Advanced Research   Doctoral Course

  • 2023   Systems Engineering Advanced Research   Doctoral Course

  • 2023   Systems Engineering Global Seminar Ⅰ   Doctoral Course

  • 2023   Systems Engineering Global Seminar Ⅰ   Doctoral Course

  • 2023   Systems Engineering Global Seminar Ⅱ   Doctoral Course

  • 2023   Systems Engineering Global Seminar Ⅱ   Doctoral Course

  • 2022   Systems Engineering Global Seminar Ⅱ   Doctoral Course

  • 2022   Systems Engineering Global Seminar Ⅰ   Doctoral Course

  • 2022   Systems Engineering Advanced Research   Doctoral Course

  • 2022   Systems Engineering Advanced Seminar Ⅱ   Doctoral Course

  • 2022   Systems Engineering Advanced Seminar Ⅰ   Doctoral Course

  • 2022   Systems Engineering Project SeminarⅡB   Master's Course

  • 2022   Systems Engineering Project SeminarⅡA   Master's Course

  • 2022   Systems Engineering Project SeminarⅠB   Master's Course

  • 2022   Systems Engineering Project SeminarⅠA   Master's Course

  • 2022   Introduction to Chemical Biology   Master's Course

  • 2022   Systems Engineering SeminarⅡB   Master's Course

  • 2022   Systems Engineering SeminarⅡA   Master's Course

  • 2022   Systems Engineering SeminarⅠB   Master's Course

  • 2022   Systems Engineering SeminarⅠA   Master's Course

  • 2021   Systems Engineering Global Seminar Ⅱ   Doctoral Course

  • 2021   Systems Engineering Global Seminar Ⅰ   Doctoral Course

  • 2021   Systems Engineering Advanced Research   Doctoral Course

  • 2021   Systems Engineering Advanced Seminar Ⅱ   Doctoral Course

  • 2021   Systems Engineering Advanced Seminar Ⅰ   Doctoral Course

  • 2021   Systems Engineering Project SeminarⅡB   Master's Course

  • 2021   Systems Engineering Project SeminarⅡA   Master's Course

  • 2021   Systems Engineering Project SeminarⅠB   Master's Course

  • 2021   Systems Engineering Project SeminarⅠA   Master's Course

  • 2021   Introduction to Chemical Biology   Master's Course

  • 2021   Systems Engineering SeminarⅡB   Master's Course

  • 2021   Systems Engineering SeminarⅡA   Master's Course

  • 2021   Systems Engineering SeminarⅠB   Master's Course

  • 2021   Systems Engineering SeminarⅠA   Master's Course

  • 2020   Systems Engineering Global Seminar Ⅱ   Doctoral Course

  • 2020   Systems Engineering Global Seminar Ⅰ   Doctoral Course

  • 2020   Systems Engineering Advanced Research   Doctoral Course

  • 2020   Systems Engineering Advanced Seminar Ⅱ   Doctoral Course

  • 2020   Systems Engineering Advanced Seminar Ⅰ   Doctoral Course

  • 2020   Systems Engineering Project SeminarⅡB   Master's Course

  • 2020   Systems Engineering Project SeminarⅡA   Master's Course

  • 2020   Systems Engineering Project SeminarⅠB   Master's Course

  • 2020   Systems Engineering Project SeminarⅠA   Master's Course

  • 2020   Introduction to Chemical Biology   Master's Course

  • 2020   Systems Engineering SeminarⅡB   Master's Course

  • 2020   Systems Engineering SeminarⅡA   Master's Course

  • 2020   Systems Engineering SeminarⅠB   Master's Course

  • 2020   Systems Engineering SeminarⅠA   Master's Course

  • 2019   Introduction to Chemical Biology   Master's Course

  • 2019   Systems Engineering Advanced Seminar Ⅱ   Doctoral Course

  • 2019   Systems Engineering Advanced Seminar Ⅱ   Doctoral Course

  • 2019   Systems Engineering Advanced Seminar Ⅰ   Doctoral Course

  • 2019   Systems Engineering Advanced Seminar Ⅰ   Doctoral Course

  • 2019   Systems Engineering Advanced Research   Doctoral Course

  • 2019   Systems Engineering Advanced Research   Doctoral Course

  • 2019   Systems Engineering SeminarⅡB   Master's Course

  • 2019   Systems Engineering SeminarⅡA   Master's Course

  • 2019   Systems Engineering SeminarⅠB   Master's Course

  • 2019   Systems Engineering SeminarⅠA   Master's Course

  • 2019   Systems Engineering Project SeminarⅡB   Master's Course

  • 2019   Systems Engineering Project SeminarⅠB   Master's Course

  • 2019   Systems Engineering Project SeminarⅠA   Master's Course

  • 2018   Systems Engineering Advanced Research   Doctoral Course

  • 2018   Systems Engineering Advanced Research   Doctoral Course

  • 2018   Systems Engineering Advanced Seminar Ⅰ   Doctoral Course

  • 2018   Systems Engineering Advanced Seminar Ⅰ   Doctoral Course

  • 2018   Systems Engineering Project SeminarⅡB   Master's Course

  • 2018   Systems Engineering Project SeminarⅡA   Master's Course

  • 2018   Systems Engineering Project SeminarⅠB   Master's Course

  • 2018   Systems Engineering Project SeminarⅠA   Master's Course

  • 2018   Systems Engineering SeminarⅡB   Master's Course

  • 2018   Systems Engineering SeminarⅡA   Master's Course

  • 2018   Systems Engineering SeminarⅠB   Master's Course

  • 2018   Systems Engineering SeminarⅠA   Master's Course

  • 2017   Systems Engineering Advanced Research   Doctoral Course

  • 2017   Systems Engineering Advanced Research   Doctoral Course

  • 2017   Systems Engineering Advanced Seminar Ⅰ   Doctoral Course

  • 2017   Systems Engineering Project SeminarⅠA   Master's Course

  • 2017   Systems Engineering SeminarⅡB   Master's Course

  • 2017   Systems Engineering SeminarⅡA   Master's Course

  • 2017   Systems Engineering SeminarⅠB   Master's Course

▼display all

Papers and Awards Received Related to Improving Education

  • 2019   グッドレクチャー賞   和歌山大学 システム工学部長   Domestic

Published Papers

  • Tripodal Quinone-Cyanine G-Quadruplex Ligands as Novel Photosensitizers on Photoinduced Cancer Cell Death

    Junya Muramoto, Takashi Sakamoto (Part: Last author, Corresponding author )

    Molecules     2024.10  [Refereed]  [Invited]

    DOI

  • Near-Infrared Fluorescent Switch-On Probe for Guanine-Quadruplex Imaging with Extremely Large Stokes Shift

    Yuka Muraoka, Junya Muramoto, Yu Yasuhara, Mayuko Kawatake, Takashi Sakamoto (Part: Last author, Corresponding author )

    Analytical Chemistry ( American Chemical Society (ACS) )  95 ( 47 ) 17162 - 17165   2023.11  [Refereed]

    DOI

  • Tripodal Quinone-cyanine Fluorescent Dye as a Tool for Analyzing Structural Dynamics of DNA Guanine-quadruplex

    Takashi Sakamoto, Zehui Yu (Part: Lead author, Corresponding author )

    Chemistry Letters ( The Chemical Society of Japan )  51 ( 12 ) 1139 - 1142   2022.12  [Refereed]

    DOI

  • Dual-Color Fluorescence Switch-On Probe for Imaging G-Quadruplex and Double-Stranded DNA in Living Cells

    Takashi Sakamoto, Zehui Yu, Yuto Otani (Part: Lead author, Corresponding author )

    Analytical Chemistry ( American Chemical Society ({ACS}) )  94 ( 10 ) 4269 - 4276   2022.03  [Refereed]

    DOI

  • Simultaneous Amino Acid Analysis Based on <sup>19</sup>F NMR Using a Modified OPA-Derivatization Method

    Takashi Sakamoto, Zhiyong Qiu, Miyu Inagaki, Kenzo Fujimoto (Part: Lead author, Corresponding author )

    Analytical Chemistry ( American Chemical Society (ACS) )  92 ( 2 ) 1669 - 1673   2019.12  [Refereed]

    DOI

  • Photo-cross-linkable artificial nucleic acid: Synthesis and properties of 3-cyanovinylcarbazole-modified nucleic acids and its photo-induced gene-silencing activity in cells

    Takashi Sakamoto, Kenzo Fujimoto (Part: Lead author )

    Synthesis of Therapeutic Oligonucleotides     171 - 186   2018.12  [Refereed]

     View Summary

    © Springer Nature Singapore Pte Ltd. 2018. The inter-strand photo-cross-linking reaction between nucleic acid strands has wide potential for regulating gene expression specifically and spatiotemporally due to its sequence specificity and high photo irradiation operability. Therefore, photo-cross-linkable artificial nucleic acids are required to be specific and effective drugs without adverse effects and also be good tools for investigating gene functions in cells. As one of the most reactive photo-cross-linkable artificial nucleic acids, in this review, 3-cyanovinylcarbazole modified oligodeoxyribonucleotides that can photo-crosslink with their complementary nucleic acid within a few seconds of photoirradiation are examined. The details of the synthetic method, properties and the applications for regulating gene expression in cells are discussed.

    DOI

  • Disassembly-driven signal turn-on probes for bimodal detection of DNA with 19F NMR and fluorescence

    Takashi Sakamoto, Daisaku Hasegawa, Kenzo Fujimoto (Part: Lead author, Corresponding author )

    Organic & Biomolecular Chemistry ( Royal Society of Chemistry ({RSC}) )    2018  [Refereed]

    DOI

  • Effect of nucleobase change on cytosine deamination through DNA photo-cross-linking reaction via 3-cyanovinylcarbazole nucleoside.

    Sethi S, Ooe M, Sakamoto T, Fujimoto K

        2017.04  [Refereed]

     View Summary

    Sethi S, Ooe M, Sakamoto T, Fujimoto K, Molecular bioSystems, 2017, 2017

    DOI

  • Characterization of human telomere RNA G-quadruplex structures in vitro and in living cells using 19F NMR spectroscopy.

    Bao HL, Ishizuka T, Sakamoto T, Fujimoto K, Uechi T, Kenmochi N, Xu Y

        2017.02  [Refereed]

     View Summary

    Bao HL, Ishizuka T, Sakamoto T, Fujimoto K, Uechi T, Kenmochi N, Xu Y, Nucleic acids research, 2017, 2017

    DOI

  • UVA-responsive Anticancer Prodrugs Based on Photoinduced Electron Injection into Oligonucleotide Having 5-Halouracils

    Fujimoto, Kenzo, Furusawa, Mirei, Nakamura, Shigetaka, Sakamoto, Takashi (Part: Last author )

    Chemistry Letters ( The Chemical Society of Japan )  45 ( 9 ) 1078 - 1080   2016.06  [Refereed]

    DOI

  • Simultaneous detection of single-nucleotide polymorphisms in a DNA bulge structure using a fluorine-modified bisbenzimide derivative

    Sakamoto, Takashi, Hasegawa, Daisaku, Fujimoto, Kenzo (Part: Lead author, Corresponding author )

    Analyst ( Royal Society of Chemistry )  141 ( 4 ) 1214 - 1217   2016.02  [Refereed]

    DOI

  • Photo-Cross-Linking Reaction in Nucleic Acids: Chemistry and Applications

    Sakamoto, Takashi, Fujimoto, Kenzo (Part: Lead author )

    Modified Nucleic Acids ( Springer )    145 - 157   2016  [Refereed]

  • RNA fluorescence in situ hybridization using 3-cyanovinylcarbazole modified oligodeoxyribonucleotides as photo-cross-linkable probes

    Fujimoto, Kenzo, Toyosato, Kei, Nakamura, Shigetaka, Sakamoto, Takashi (Part: Last author )

    Bioorganic & Medicinal Chemistry Letters ( Pergamon )  26 ( 21 ) 5312 - 5314   2016  [Refereed]

    DOI

  • Critical effect of base pairing of target pyrimidine on the interstrand photo-cross-linking of DNA via 3-cyanovinylcarbazole nucleoside

    Sakamoto, Takashi, Ooe, Minako, Fujimoto, Kenzo (Part: Lead author )

    Bioconjugate chemistry ( ACS Publications )  26 ( 8 ) 1475 - 1478   2015  [Refereed]

    DOI

  • DNA photo-cross-linking using 3-cyanovinylcarbazole modified oligonucleotide with threoninol linker

    Sakamoto, Takashi, Tanaka, Yuya, Fujimoto, Kenzo (Part: Lead author )

    Organic letters ( ACS Publications )  17 ( 4 ) 936 - 939   2015  [Refereed]

    DOI

  • Changing blue fluorescent protein to green fluorescent protein using chemical RNA editing as a novel strategy in genetic restoration

    Vu, Luyen T, Nguyen, Thanh TK, Alam, Shafiul, Sakamoto, Takashi, Fujimoto, Kenzo, Suzuki, Hitoshi, Tsukahara, Toshifumi

    Chemical biology & drug design ( Wiley Online Library )  86 ( 5 ) 1242 - 1252   2015  [Refereed]

    DOI

  • Fluorine-modified bisbenzimide derivative as a molecular probe for bimodal and simultaneous detection of DNAs by 19 F NMR and fluorescence

    Sakamoto, Takashi, Hasegawa, Daisaku, Fujimoto, Kenzo (Part: Lead author, Corresponding author )

    Chemical Communications ( Royal Society of Chemistry )  51 ( 42 ) 8749 - 8752   2015  [Refereed]

    DOI

  • Short oligonucleotide prodrug having 5-fluoro and 5-iodouracil inhibits the proliferation of cancer cells in a photo-responsive manner

    Fujimoto, Kenzo, Takematsu, Yu-ki, Shigeno, Atsuo, Furusawa, Mirei, Sakamoto, Takashi (Part: Last author )

    Bioorganic & medicinal chemistry letters ( Elsevier )  24 ( 16 ) 3736 - 3738   2014  [Refereed]

    DOI

  • Photo-regulation of constitutive gene expression in living cells by using ultrafast photo-cross-linking oligonucleotides

    Sakamoto, Takashi, Shigeno, Atsuo, Ohtaki, Yuichi, Fujimoto, Kenzo (Part: Lead author )

    Biomaterials Science ( Royal Society of Chemistry )  2 ( 9 ) 1154 - 1157   2014  [Refereed]

    DOI

  • Details of the ultrafast DNA photo-cross-linking reaction of 3-cyanovinylcarbazole nucleoside: cis--trans isomeric effect and the application for SNP-based genotyping

    Fujimoto, Kenzo, Yamada, Asuka, Yoshimura, Yoshinaga, Tsukaguchi, Tadashi, Sakamoto, Takashi (Part: Last author )

    Journal of the American Chemical Society ( ACS Publications )  135 ( 43 ) 16161 - 16167   2013  [Refereed]

    DOI

  • Diamine Derivatives Accelerate Photochemical C→ U Transition in DNA Double Strand

    Fujimoto, Kenzo, Futamura, Daiki, Sakamoto, Takashi (Part: Last author )

    Chemistry Letters ( The Chemical Society of Japan )  42 ( 3 ) 289 - 291   2013  [Refereed]

    DOI

  • Quick and reversible photocrosslinking reaction of 3-cyanovinylcarbazole nucleoside in a DNA triplex

    Fujimoto, Kenzo, Yoshinaga, Hiroki, Yoshio, Yasumasa, Sakamoto, Takashi (Part: Last author )

    Organic & biomolecular chemistry ( Royal Society of Chemistry )  11 ( 31 ) 5065 - 5068   2013  [Refereed]

    DOI

  • Quick, selective and reversible photocrosslinking reaction between 5-methylcytosine and 3-cyanovinylcarbazole in DNA double strand

    Fujimo, Kenzo, Konishi-Hiratsuka, Kaoru, Sakamoto, Takashi (Part: Last author )

    International journal of molecular sciences ( Multidisciplinary Digital Publishing Institute )  14 ( 3 ) 5765 - 5774   2013  [Refereed]

    DOI

  • Geometric Effect on the Photocrosslinking Reaction between 3-Cyanovinylcarbazole Nucleoside and Pyrimidine Base in DNA/RNA Heteroduplex

    Fujimoto, Kenzo, Kishi, Satomi, Sakamoto, Takashi (Part: Last author )

    Photochemistry and photobiology ( Wiley Online Library )  89 ( 5 ) 1095 - 1099   2013  [Refereed]

    DOI

  • Development about photochemical DNA and RNA crosslinking via 3-cyanovinylcarbazole

    Satomi Kishi, Daiki Futamura, Kaoru Hiratsuka, Takashi Sakamoto, Kenzo Fujimoto

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY ( AMER CHEMICAL SOC )  243   2012.03  [Refereed]

  • Regulation of gene expression by photocrosslinking oligonucleotide containing of carbazole

    Atsuo Shigeno, Sakamoto Takashi, Kenzo Fujimoto

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY ( AMER CHEMICAL SOC )  243   2012.03  [Refereed]

  • Quick regulation of mRNA functions by a few seconds of photoirradiation

    Shigeno, Atsuo, Sakamoto, Takashi, Yoshimura, Yoshinaga, Fujimoto, Kenzo

    Organic & biomolecular chemistry ( Royal Society of Chemistry )  10 ( 38 ) 7820 - 7825   2012  [Refereed]

    DOI

  • Specific and reversible photochemical labeling of plasmid DNA using photoresponsive oligonucleotides containing 3-cyanovinylcarbazole

    Fujimoto, Kenzo, Hiratsuka-Konishi, Kaoru, Sakamoto, Takashi, Ohtake, Tomoko, Shinohara, Ken-ichi, Yoshimura, Yoshinaga

    Molecular BioSystems ( Royal Society of Chemistry )  8 ( 2 ) 491 - 494   2012  [Refereed]

    DOI

  • Signal turn-on probe for nucleic acid detection based on 19 F nuclear magnetic resonance

    Sakamoto, Takashi, Shimizu, Yu-ki, Sasaki, Jun, Hayakawa, Hikaru, Fujimoto, Kenzo (Part: Lead author )

    Bioorganic & medicinal chemistry letters ( Elsevier )  21 ( 1 ) 303 - 306   2011  [Refereed]

    DOI

  • 5-Methylcytosine selective photoligation using photoresponsive oligonucleotides containing various 5-vinyl-2′-deoxyuridines having an aromatic group

    Sakamoto, Takashi, Ami, Takehiro, Fujimoto, Kenzo (Part: Lead author )

    Chemistry Letters ( The Chemical Society of Japan )  41 ( 1 ) 47 - 49   2011  [Refereed]

    DOI

  • Development of a Potassium Ion Sensor for 19F Magnetic Resonance Chemical Shift Imaging Based on Fluorine-labeled Thrombin Aptamer

    Sakamoto, Takashi, Hayakawa, Hikaru, Fujimoto, Kenzo (Part: Lead author )

    Chemistry Letters ( The Chemical Society of Japan )  40 ( 7 ) 720 - 721   2011  [Refereed]

    DOI

  • 1P346 DNA computing based on photochemical DNA manipulation(Miscellaneous topics,The 48th Annual Meeting of the Biophysical Society of Japan)

    Ogasawara Shinji, Sakamoto Takashi, Nakamura Shigetaka, Fujimoto Kenzo

    Seibutsu Butsuri ( The Biophysical Society of Japan General Incorporated Association )  50 ( 2 ) S81   2010

    DOI

  • Site-specific photochemical RNA editing

    Fujimoto, Kenzo, Konishi-Hiratsuka, Kaoru, Sakamoto, Takashi, Yoshimura, Yoshinaga

    Chemical Communications ( Royal Society of Chemistry )  46 ( 40 ) 7545 - 7547   2010  [Refereed]

    DOI

  • Site-Specific Cytosine to Uracil Transition by Using Reversible DNA Photo-crosslinking

    Fujimoto, Kenzo, Konishi-Hiratsuka, Kaoru, Sakamoto, Takashi, Yoshimura, Yoshinaga

    ChemBioChem ( Wiley Online Library )  11 ( 12 ) 1661 - 1664   2010  [Refereed]

    DOI

  • Molecular recognition, fluorescence sensing, and biological assay of phosphate anion derivatives using artificial Zn (II)--Dpa complexes

    Sakamoto, Takashi, Ojida, Akio, Hamachi, Itaru (Part: Lead author )

    Chemical Communications ( Royal Society of Chemistry )  ( 2 ) 141 - 152   2009  [Refereed]

    DOI

  • Self-assembling nanoprobes that display off/on 19F nuclear magnetic resonance signals for protein detection and imaging

    Takaoka, Yousuke, Sakamoto, Takashi, Tsukiji, Shinya, Narazaki, Michiko, Matsuda, Tetsuya, Tochio, Hidehito, Shirakawa, Masahiro, Hamachi, Itaru

    Nature chemistry ( Nature Publishing Group )  1 ( 7 ) 557 - 561   2009  [Refereed]

    DOI

  • Fluorescent BODIPY-based Zn (II) complex as a molecular probe for selective detection of neurofibrillary tangles in the brains of Alzheimer’s disease patients

    Ojida, Akio, Sakamoto, Takashi, Inoue, Masa-aki, Fujishima, Sho-hei, Lippens, Guy, Hamachi, Itaru

    Journal of the American Chemical Society ( ACS Publications )  131 ( 18 ) 6543 - 6548   2009  [Refereed]

    DOI

  • Real-time fluorescence monitoring of GSK3$β$-catalyzed phosphoryation by use of a BODIPY-based Zn (II)--Dpa chemosensor

    Sakamoto, Takashi, Inoue, Masa-aki, Ojida, Akio, Hamachi, Itaru (Part: Lead author )

    Bioorganic & medicinal chemistry letters ( Elsevier )  19 ( 15 ) 4175 - 4177   2009  [Refereed]

    DOI

  • Microarray-based label-free detection of RNA using bispyrene-modified 2′-O-methyl oligoribonucleotide as capture and detection probe

    Sakamoto, Takashi, Kobori, Akio, Murakami, Akira (Part: Lead author )

    Bioorganic & medicinal chemistry letters ( Elsevier )  18 ( 8 ) 2590 - 2593   2008  [Refereed]

    DOI

  • Transcriptome analysis device based on liquid phase detection by fluorescently labeled nucleic acid probes

    Yokokawa, Ryuji, Tamaoki, Soichiro, Sakamoto, Takashi, Murakami, Akira, Sugiyama, Susumu

    Biomedical microdevices ( Springer )  9 ( 6 ) 869 - 875   2007  [Refereed]

    DOI

  • Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen

    Munaka, Tatsuya, Abe, Hirohisa, Kanai, Masaki, Sakamoto, Takashi, Nakanishi, Hiroaki, Shoji, Shuichi, Kimura, Shinya, Maekawa, Taira, Murakami, Akira

    Analyst ( Royal Society of Chemistry )  132 ( 6 ) 512 - 514   2007  [Refereed]

    DOI

  • Homogeneous Fluorescence Assays for RNA Diagnosis by Pyrene-Conjugated 2′-O-Methyloligoribonucleotides

    Sakamoto, Takashi, Kobori, Akio, Shigezawa, Masako, Amitani, Yasutaka, Higuchi, Maiko, Murakami, Akira (Part: Lead author )

    Nucleosides, Nucleotides, and Nucleic Acids ( Taylor \& Francis )  26 ( 10-12 ) 1659 - 1664   2007  [Refereed]

    DOI

  • Highly-sensitive detection of immunoreactions by time-resolved fluorescence anisotropy analysis

    Takashi Sakamoto, Nobuyuki Fujihara, Akio Kobori, Akira Murakami, Miharu Kishi, Koichi Yamagata

    Polymer Preprints, Japan   55 ( 2 ) 4933   2006

     View Summary

    Long-lifetime fluorophore is useful for the highly-sensitive detection of immunoreactions based on time-resolved fluorescence anisotropy analysis. We focused on pyrene-β-cyclodextrin conjugate (Py-β-CD) as a long-lifetime fluorescent label. Py-β-CD-NHS-ester was used to label human serum albumin (HSA). The steady-state fluorescence anisotropy of Py-β-CD-labeled HSA (Py-β-CD-HSA) was measured in the presence of various proteins. The fluorescence anisotropy of Py-β-CD-HSA increased upon the addition of anti-HSA monoclonal IgG or anti-HSA polyclonal IgG. On the other hand, the addition of anti-IgG IgG or HSA did not affect the fluorescence anisotropy of Py-β-CD-HSA. Time-resolved fluorescence anisotropy analysis of the Py-β-CD-HSA was carried out in the presence or absence of anti-HSA polyclonal IgG. The fluorescence anisotropy of the Py-β-CD-HSA from 0 to 200 nsec after pulse excitation significantly increased by the addition of anti-HSA polyclonal IgG. These results indicated that Py-β-CD-HSA bound to its specific IgG and that Py-β-CD-HSA was useful for highly-sensitive detection of immunoreactions based on time-resolved fluorescence anisotropy analysis.

  • Properties of novel antisense oligonucleotides containing 2′-O-modified adenosine with a photo-reactive group

    Higuchi Maiko, Sakamoto Takashi, Kobori Akio, Murakami Akira

    Nucleic Acids Symposium Series   50 ( 1 ) 301 - 2   2006  [Refereed]

     View Summary

    In order to enhance the efficiency of antisense molecules for target RNA regulation, a novel photo-reactive antisense oligonucleotide was developed. We designed and synthesized a photo-reactive antisense oligonucleotide containing an adenosine in which 2'-OH was modified with 4,5',8-trimethylpsoralen (Ps) via an ethoxymethylene linkage (2'-Ps-eom). We evaluated the photo-cross-linking efficiency and sequence specificity toward complementary RNA (match-RNA). 2'-Ps-eom was selectively photo-cross-linked to the match-RNA. The photo-cross-linking efficiency was about 75% upon UVA-irradiation (365 nm) for 10 min. Previously, we reported oligonucleotides that had an adenosine anchoring Ps at 2'-O-position via a methylene linkage (2'-Ps-met). The photo-cross-linking efficiency of 2'-Ps-met and match-RNA was about 35% upon UVA-irradiation for 120 min. The photo-cross-linking efficiency of 2'-Ps-eom was dramatically enhanced in comparison with the one of 2'-Ps-met.

  • Solid-phase detection of RNA using bispyrene-modified RNA probe

    Sakamoto Takashi, Kobori Akio, Murakami Akira (Part: Lead author )

    Nucleic Acids Symposium Series   50 ( 1 ) 215 - 6   2006  [Refereed]

     View Summary

    Bispyrene modified 2'-O-methyl oligoRNA (OMUpy2), which is a useful fluorescent probe for specific RNA detection, was immobilized on a glass substrate via various linker molecules to develop a more convenient RNA detection chip for gene expression study. As the fluorescence intensity of OMUpy2 enhanced greatly when it hybridized with its complementary RNA, it was expected that RNA detection by OMUpy2-immobilized chip (RNA-chip) required neither fluorescent labels to target nucleic acid nor washing protocol after the hybridization. As the linker molecules, oligo(dT), agarose, and poly(ethylene glycol) (PEG) were chosen, and the fluorescence from the chip was measured by a fluorescence microscope. Results indicated that the RNA-chip was able to detect the complementary oligoribonucleotide (cORN) without target labeling and washing protocols. Furthermore, the background emission was reduced as the length of the linker increased. In the case of PEG linker, 25-fold enhancement of the fluorescence intensity of the OMUpy2 was observed upon the addition of cORN.

  • Monitoring cellular events in living mast cells stimulated with an extremely small amount of fluid on a microchip

    Munaka, Tatsuya, Abe, Hirohisa, Kanai, Masaki, Sakamoto, Takashi, Nakanishi, Hiroaki, Yamaoka, Tetsuji, Shoji, Shuichi, Murakami, Akira

    Japanese journal of applied physics ( IOP Publishing )  45 ( 7L ) L634   2006  [Refereed]

    DOI

  • Real-time monitoring of antibody secretion from hybridomas on a microchip by time-resolved luminescence anisotropy analysis

    Munaka, Tatsuya, Abe, Hirohisa, Kanai, Masaki, Sakamoto, Takashi, Nakanishi, Hiroaki, Yamaoka, Tetsuji, Shoji, Shuichi, Murakami, Akira

    Analytical biochemistry ( Elsevier )  353 ( 1 ) 1 - 6   2006  [Refereed]

    DOI

  • Development of the cell analysis chip (II)

    Tatsuya Munaka, Masaki Kanai, Hirohisa Abe, Hiroaki Nakanishi, Takashi Sakamoto, Akio Kobori, Tetsuji Yamaoka, Shuichi Shoji, Akira Murakami

    Polymer Preprints, Japan   54 ( 1 ) 2329   2005  [Refereed]

     View Summary

    In recent years, cell analysis has attracted increased attention in bioanalytical and biotechnological applications. On the other hand, microchip techniques, which are called micro total analysis systems (μTAS), have been developed especially in chemical and biochemical analysis fields. We took advantage of a feature of μ TAS and developed a system that can detect a small amount of the protein released by a cell without bound/free (B/F) separation protocol and with high sensitivity. In this report, we describe the continuous monitoring of immunoglobulin G (lgG) production of hybridoma cells stimulated by the serum.

  • Analytical method for estimation of kinetics of oligonucleotide/RNA hybridization using fluorescence depolarization spectroscopy

    Sakamoto, Takashi, Mahara, Atsushi, Iwase, Reiko, Yamaoka, Tetsuji, Murakami, Akira (Part: Lead author )

    Analytical biochemistry ( Academic Press )  340 ( 2 ) 369 - 372   2005  [Refereed]

    DOI

  • Transcriptome Analyzing Chip Based on Liquid-phase Detection Using Fluorescence Labeled Nucleic Acid Probe

    Tamaoki S, Sugiyama S, Sakamoto T, Murakami A

    PROCEEDINGS OF THE SENSOR SYMPOSIUM ON SENSORS MICROMACHINES AND APPLIED SYSTEMS   22   270   2005  [Refereed]

  • Evaluation of dynamic features of Escherichia coli 16S ribosomal RNA in homogeneous physiological solution

    Sakamoto, Takashi, Mahara, Atsushi, Yamagata, Koichi, Iwase, Reiko, Yamaoka, Tetsuji, Murakami, Akira (Part: Lead author )

    Biophysical journal ( Elsevier )  89 ( 6 ) 4122 - 4128   2005  [Refereed]

    DOI

  • Luminescence anisotropy-based detection of nucleic acids and proteins using long-lifetime Ru(II) complex as a luminescent label.

    Takashi Sakamoto, Atsushi Mahara, Kobori Akio, Tetsuji Yamaoka, Akira Murakami (Part: Lead author )

    Nucleic acids symposium series (2004)   49 ( 49 ) 203 - 204   2005  [Refereed]

     View Summary

    Luminescence anisotropy-based methods are powerful tool for the detection of biomolecules in homogeneous physiological media without Bound/Free separation. However, analyses based on the method are always disturbed by the autofluorescence from biological specimen. This disturbance limits the sensitivity of the method. To improve the detection limit of the method, a long-lifetime luminophore was adopted as the label. Because of the lifetime of the autofluorescence is below 10 ns, it is expected that the use of the long-lifetime luminophore enables us to avoid the disturbance by adopting time-resolved luminescence anisotropy measurement. To verify this concept, oligodeoxyribonucleotides and Staphylococcus aureus protein A were labeled with Ru(II) complex (tau approximately 500 nsec) and used for the detection of Escherichia coli 16S ribosomal RNA (16S rRNA) and immunoglobulin G (IgG), respectively. Results indicated that the methods were useful for the detection of rRNA and IgG without disturbance of the autofluorescence.

  • Time-resolved luminescence anisotropy-based detection of immunoglobulin G using long-lifetime Ru (II) complex-labeled protein A

    Sakamoto, Takashi, Mahara, Atsushi, Munaka, Tatsuya, Yamagata, Koich, Iwase, Reiko, Yamaoka, Tetsuji, Murakami, Akira (Part: Lead author )

    Analytical biochemistry ( Academic Press )  329 ( 1 ) 142 - 144   2004  [Refereed]

    DOI

  • Transcriptome analysis using fluorescence-labeled oligonucleotide.

    Naohiko Shimada, Atsushi Mahara, Takashi Sakamoto, Tetsuji Yamaoka, Akira Murakami

    Nucleic acids symposium series (2004)   48 ( 48 ) 301 - 302   2004  [Refereed]

     View Summary

    In our previous studies, the fluorescence intensity at 480 nm of the bispyrene-labeled 2'-O-methyl-oligoribonucleotide (OMUpy2) was drastically enhanced only when it was hybridized with its complementary RNA. In this study, we demonstrated the potential of the OMUpy2 for transcriptome analysis in the cell free system and in the living cell. For the analysis in the cell free system, the micro-chamber was designed and used to evaluate the detection limit of the target RNA. The detection limit of oligo-RNA was approximately 15 fmol. For the analysis of the living cells, C-OMUpy2 was introduced to the cervical carcinoma cells, C4II, and the fluorescence from C-OMUpy2 was monitored. The fluorescence from the cells began to be observed 5 min after the serum stimulation, and the fluorescence was decreased after 20 min. These results seemed to be consistent with the results from the RT-PCR analysis and suggested that OMUpy2 could be a useful tool for the transcriptome analysis.

  • In situ Cell Monitoring on a Microchip using Time-Resolved Fluorescence Anisotropy Analysis

    Sakamoto Takashi, Mahara Atsushi, Yamayoshi Asako

    Seibutsu Butsuri ( The Biophysical Society of Japan General Incorporated Association )  43   S222   2003.10  [Refereed]

    DOI

  • RNAの機能・構造解析に用いる螢光RNAプローブの開発

    村上 章, 坂本 隆, 馬原 淳

    化学と生物 ( Japan Society for Bioscience, Biotechnology, and Agrochemistry )  41 ( 8 ) 549 - 556   2003.08

    DOI

  • Detection of acceptor sites for antisense oligonucleotides on native folded RNA by fluorescence-labeled oligonucleotide.

    Atsushi Mahara, Takashi Sakamoto, Tatsuya Munaka, Reiko Iwase, Tetsuji Yamaoka, Akira Murakami

    Nucleic acids research. Supplement (2001)   3 ( 3 ) 73 - 74   2003  [Refereed]

     View Summary

    Pyrene-labeled 2'-O-methyloligoribonucleotide (OMUpy) and 5'-Ru(II) complex labeled oligodeoxyribonucleotie (Ru-probe) were prepared as the fluorescence probes to detect acceptor sites of antisense molecules on native folded RNA under the physiological condition. OMUpy showed the remarkable increase of the fluorescence intensity (334-fold at 375 nm) only when hybridized with complementary oligoRNA. When OMUpy was applied to E. coli 16S-rRNA, the fluorescence intensities were increased in a sequence specific manner. The rotational correlation time of Ru-probe complementary to 16S-rRNA were largely dependent on the sequence of Ru-probe. These results suggest that the accessibility of the antisense molecules for RNA can be evaluated by those fluorescent probes.

  • Detection of acceptor sites for antisense oligonucleotides on native folded RNA by fluorescence spectroscopy

    Mahara, Atsushi, Iwase, Reiko, Sakamoto, Takashi, Yamaoka, Tetsuji, Yamana, Kazushige, Murakami, Akira

    Bioorganic & medicinal chemistry ( Elsevier )  11 ( 13 ) 2783 - 2790   2003  [Refereed]

    DOI

  • Bispyrene-conjugated 2'-O-methyloligonucleotide as a highly specific RNA-recognition probe.

    Atsushi Mahara, Reiko Iwase, Takashi Sakamoto, Kazushige Yamana, Tetsuji Yamaoka, Akira Murakami

    Angewandte Chemie (International ed. in English)   41 ( 19 ) 3648 - 50   2002.10

  • Bispyrene-Conjugated 2′-O-Methyloligonucleotide as a Highly Specific RNA-Recognition Probe

    Mahara, Atsushi, Iwase, Reiko, Sakamoto, Takashi, Yamana, Kazushige, Yamaoka, Tetsuji, Murakami, Akira

    Angewandte Chemie ( WILEY-VCH Verlag )  114 ( 19 ) 3800 - 3802   2002  [Refereed]

    DOI

  • Study on structure of ribosomal RNA by time-resolved luminescence anisotropy analysis.

    T. Sakamoto, A. Mahara, R. Iwase, T. Yamaoka, A. Murakami (Part: Lead author )

    Nucleic acids research. Supplement (2001)   1 ( 1 ) 143 - 144   2001  [Refereed]

     View Summary

    Evaluation of the folded structure of E.coli 16S-rRNA was performed using 5'-Ru(II) complex labeled oligodeoxyribonucleotide (Ru-probe) as the probe. This probe could evaluate the dynamic manner of the binding site of Ru-probe by using the time-resolved luminescence anisotropy technique. It was found that the rotational manner of Ru-probe in the presence of 16S-rRNA was markedly dependent on the oligonucleotide sequence, indicating that the microenvironments around the Ru-probes were largely different.

    DOI

▼display all

Books etc

  • Live-Cell RNA Imaging –Methods and Protocols, Ed. Shin-ichi Sato (Ed), Methods in Molecular Biology

    Takashi Sakamoto( Part: Contributor,  Work: Live-Cell Imaging of RNA G-Quadruplex with a Dual-Color Fluorescence Switch-on Probe)

    Springer Nature  2025.01  ISBN: 9781071642474

  • Modified nucleic acids

    Nakatani, Kazuhiko, Tor, Yitzhak

    Springer  2016  ISBN: 9783319271095

  • 新しいDNAチップの科学と応用

    関根, 光雄, 講談社サイエンティフィク

    講談社  2007.07  ISBN: 9784061538641

Misc

  • Regulating the binding selectivity of dual-color fluorescence switch-on probe that responses with duplex and quadruplex DNA

    村岡優香, 安原優, 岩井まり奈, 坂本隆, 坂本隆

    日本化学会春季年会講演予稿集(Web)   103rd   2023

  • Development of photochromic DNA imaging probe whose fluorescence properties can be regulated by photoirradiation

    三輪好輝, 坂本隆

    日本化学会春季年会講演予稿集(Web)   102nd   2022

  • Simultaneous detection of double-stranded and G4 DNAs using a tripodal quinone-cyanine dye

    坂本隆, 坂本隆, YU Zehui, 大谷悠人

    日本化学会春季年会講演予稿集(Web)   101st   2021

  • Development of a thin layer made of fluorescence turn-on DNA probe and the application for micro-RNA detections

    坂本隆

    サムコ科学技術振興財団研究報告集   3   2021

  • Label-free in situ Monitoring of DNA Hybridization Chain Reaction Using Sequence-Selective Interaction of Groove Binders with Double-Stranded DNAs

    坂本隆, 山田陸斗

    日本化学会春季年会講演予稿集(CD-ROM)   100th   2020

  • ヘキスト系色素の非標準構造DNAに対する光反応の検討

    三輪好輝, 瀬戸上慧, 坂本隆

    日本化学会春季年会講演予稿集(CD-ROM)   99th   2019

  • DNAマイナーグルーブ結合型蛍光色素を用いたハイブリダイゼーション連鎖反応のラベルフリー検出

    山田陸斗, 坂本隆, 坂本隆

    日本化学会春季年会講演予稿集(CD-ROM)   99th   2019

  • <sup>19</sup>F NMRを用いた核酸等生体分子の同時一斉計測技術の開発

    坂本隆, 長谷川大策, QIU Zhiyong, 藤本健造

    バイオ・高分子シンポジウム講演要旨集   27th   2017

  • フッ素標識ヘキストプローブを用いた<sup>19</sup>F NMRによるDNA検出

    坂本隆, 長谷川大策, 藤本健造

    バイオ・高分子シンポジウム講演要旨集   26th   2016

  • <sup>19</sup>F NMRを用いたDNA1塩基多型の同時一斉解析

    坂本隆, 長谷川大策, 藤本健造

    日本化学会春季年会講演予稿集(CD-ROM)   96th   2016

  • 光か学的なRNA架橋反応を用いた新規FISH法の開発

    中村重孝, 豊里慧, 狩野千波, 坂本隆, 藤本健造

    日本分子生物学会年会プログラム・要旨集(Web)   39th   2016

  • フッ素修飾ビスベンズイミド誘導体を用いた<sup>19</sup>F NMRによるDNAのOFF/ON検出

    長谷川大策, 坂本隆, 藤本健造

    日本化学会春季年会講演予稿集(CD-ROM)   96th   2016

  • グリシドール骨格を有するDNA光架橋素子の合成とその機能評価

    望月卓海, 坂本隆, 藤本健造

    日本化学会春季年会講演予稿集(CD-ROM)   96th   2016

  • 3-シアノビニルカルバゾールを含む光応答性オリゴDNAプローブを用いた新規FISH法の開発

    豊里慧, 坂本隆, 藤本健造

    日本化学会春季年会講演予稿集(CD-ROM)   96th   2016

  • Hoechst33258骨格を有するDNA検出<sup>19</sup>F核磁気共鳴プローブの開発

    坂本隆, 長谷川大策, 藤本健造

    日本化学会講演予稿集   95th ( 3 )   2015

  • 可逆的光架橋反応を用いたシトシンの脱アミノ化反応における相補塩基の効果

    大江美成子, 坂本隆, 藤本健造

    日本化学会講演予稿集   95th ( 3 )   2015

  • <sup>19</sup>F核磁気共鳴による核酸検出を可能とする新規分子プローブの開発

    坂本隆, 長谷川大策, 藤本健造

    バイオ・高分子シンポジウム講演要旨集   25th   2015

  • マルチモードイメージングを目指した新規核酸イメージングプローブの開発

    脇田和明, 坂本隆, 藤本健造, 藤本健造

    日本化学会講演予稿集   94th ( 3 )   2014

  • 高速光架橋性アンチセンス核酸による定常発現遺伝子の細胞内制御

    坂本隆, 滋野敦夫, 大滝優一, 藤本健造, 藤本健造

    アンチセンスシンポジウム講演要旨集   24th   2014

  • 3-シアノビニルカルバゾールヌクレオシドによるDNA光クロスリンク反応におけるピリミジン5位の置換基効果

    村松穂高, 中村重孝, 坂本隆, 藤本健造, 藤本健造

    日本化学会講演予稿集   94th ( 3 )   2014

  • 3-シアノビニルカルバゾールとトレオニノール骨格を有する新規DNA・RNA光クロスリンカーの開発

    田中佑弥, 坂本隆, 藤本健造, 藤本健造

    日本化学会講演予稿集   94th ( 3 )   2014

  • 光架橋性オリゴヌクレオチドによる細胞内遺伝子発現制御法の開発

    坂本隆, 滋野敦夫, 大滝優一, 藤本健造, 藤本健造

    日本光医学・光生物学会   36th   2014

  • カルバゾール誘導体を含む光応答性アンチセンス核酸を用いた遺伝子発現制御

    滋野敦夫, 坂本隆, 藤本健造

    日本化学会講演予稿集   93rd ( 3 )   2013

  • シュードウリジンに対する可逆的光架橋反応の開発

    岸里美, 坂本隆, 藤本健造

    日本化学会講演予稿集   93rd ( 3 )   2013

  • 三重鎖DNA中における高速光架橋反応開発

    吉永広樹, 坂本隆, 藤本健造

    日本化学会講演予稿集   93rd ( 3 )   2013

  • 2重鎖DNA光クロスリンク反応に関する反応解析

    山田明日香, 坂本隆, 吉村嘉永, 藤本健造

    日本化学会講演予稿集   93rd ( 3 )   2013

  • 高速光架橋能をもつアンチセンス核酸による細胞内遺伝子発現制御

    坂本隆, 滋野敦夫, 大滝優一, 藤本健造, 藤本健造

    アンチセンスシンポジウム講演要旨集   22nd   2012

  • カルバゾール誘導体を用いたDNA光架橋反応機構解析

    山田明日香, 坂本隆, 吉村嘉永, 池田真由美, 藤本健造, 藤本健造

    高分子学会北陸支部研究発表講演会講演要旨集   61st   2012

  • ビニルカルバゾール含有光応答性人工核酸を用いた光化学的遺伝子発現制御

    滋野敦夫, 坂本隆, 藤本健造, 藤本健造

    アンチセンスシンポジウム講演要旨集   22nd   2012

  • カルバゾール誘導体によるRNAへの光架橋反応を用いた光化学的な遺伝子発現制御

    大滝優一, 滋野敦夫, 坂本隆, 藤本健造

    日本分子生物学会年会プログラム・要旨集(Web)   35th   2012

  • 三重鎖DNA中における高速光架橋反応開発

    吉永広樹, 吉尾泰政, 坂本隆, 藤本健造, 藤本健造

    高分子学会北陸支部研究発表講演会講演要旨集   61st   2012

  • 高速光架橋能をもつアンチセンス核酸による遺伝子発現制御

    大滝優一, 滋野敦夫, 坂本隆, 藤本健造, 藤本健造

    高分子学会北陸支部研究発表講演会講演要旨集   61st   2012

  • Pseudouridineに対する可逆的光架橋反応の開発

    岸里美, 坂本隆, 藤本健造, 藤本健造

    高分子学会北陸支部研究発表講演会講演要旨集   61st   2012

  • <sup>19</sup>F核磁気共鳴による生体分子イメージングを指向したフッ素修飾核酸プローブの合成

    坂本隆, 早川輝, 清水勇喜, 佐々木淳, 藤本健造, 藤本健造

    有機合成化学セミナー講演予稿集   28th   2011

  • カリウムイオンをセンシングする<sup>19</sup>F MRI用造影プローブの開発

    早川輝, 佐々木淳, 坂本隆, 藤本健造, 藤本健造

    日本化学会バイオテクノロジー部会シンポジウム講演要旨集   14th   2011

  • 光クロスリンクを用いた選択的核酸増幅法の開発

    渡邊誉, 坂本隆, 藤本健造

    日本化学会講演予稿集   91st ( 3 )   2011

  • フッ素化合物修飾オリゴDNAを用いた<sup>19</sup>FNMRによる核酸の検出

    坂本隆, 清水勇喜, 佐々木淳, 早川輝, 藤本健造, 藤本健造

    日本化学会バイオテクノロジー部会シンポジウム講演要旨集   14th   2011

  • カルバゾール誘導体を含む光架橋性アンチセンス核酸による遺伝子発現制御

    滋野敦夫, 坂本隆, 藤本健造, 藤本健造

    日本化学会バイオテクノロジー部会シンポジウム講演要旨集   14th   2011

  • カリウムイオンをセンシングする19F MRI用造影プローブの開発

    早川輝, 坂本隆, 藤本健造

    日本化学会講演予稿集   91st ( 3 )   2011

  • 核酸のIn vivoイメージングを目指したフッ素核磁気共鳴OFF/ONプローブの開発:マルチフッ素ラベリングによる検出感度改善

    坂本隆, 清水勇喜, 佐々木淳, 早川輝, 藤本健造

    日本化学会講演予稿集   91st ( 3 )   2011

  • 光化学的なDNA及びRNAピンポイント編集

    二村大樹, 平塚薫, 吉村嘉永, 坂本隆, 藤本健造

    日本化学会講演予稿集   91st ( 3 )   2011

  • 可逆的DNA光クロスリンクオリゴによるDNAナノ構造の安定制御

    中村重孝, 坂本隆, 藤本健造

    日本化学会講演予稿集   91st ( 3 )   2011

  • 核酸のin vivo検出・イメージングのための<sup>19</sup>F核磁気共鳴シグナルOff/Onプローブ

    坂本隆, 清水勇喜, 佐々木淳, 藤本健造

    日本化学会講演予稿集   90th ( 3 )   2010

  • 遺伝子発現のin vivoイメージングを目指したフッ素核磁気共鳴プローブの開発

    坂本隆, 清水勇希, 佐々木淳, 早川輝, 藤本健造

    日本化学会バイオテクノロジー部会シンポジウム講演要旨集   13th   2010

  • シアノビニルカルバゾール修飾オリゴDNAを用いた光化学的DNA編集

    坂本隆, 池田真由美, 二村大樹, 吉村嘉永, 平塚薫, 藤本健造

    日本化学会バイオテクノロジー部会シンポジウム講演要旨集   13th   2010

  • Fluorescence anisotropy-based detection of immunoglobulin G using a long-lifetime fluorophore

    坂本隆, 務中達也, 馬原淳, 山形浩一, 山岡哲二, 村上章

    日本化学会講演予稿集   84th ( 2 )   2004

  • マイクロチップを用いた蛍光時間分解偏光解消法による細胞機能の経時的解析

    務中達也, 叶井正樹, 藤山陽一, 阿部浩久, 中西博昭, 坂本隆, 馬原淳, 山岡哲二, 村上章

    バイオ・高分子シンポジウム講演要旨集   14th   2004

  • Development of long-lifetime Ru(II) complex labeled oligonucleotide for analysis of RNA structure.

    坂本隆, 馬原淳, 岩瀬礼子, 山岡哲二, 村上章

    日本化学会講演予稿集   79th ( 2 )   2001

  • 長寿命発光能を有するRu(II)錯体を用いたRNA高次構造解析

    坂本隆, 馬原淳, 岩瀬礼子, 山岡哲二, 村上章

    高分子学会予稿集   50 ( 5 )   2001

▼display all

Conference Activities & Talks

  • 3脚型キノン-シアニンG4核酸リガンドの光増感活性によるガン細胞選択的アポトーシス誘導

    村元順哉, 坂本隆

    2024年度北陸地区講演会と研究発表会  2024.11.29  

  • 新規検出試薬を用いたグアニン四重鎖の解析

    成田侑介, 平川哲央, 津崎諒, 中久保政一, 石山宗孝, 坂本 隆

    第47回日本分子生物学会年会  2024.11.28  

  • 3脚型キノンーシアニン色素の光増感活性によるガン細胞選択的アポトーシス誘導

    村元順哉, 坂本隆

    第18回バイオ関連化学シンポジウム  2024.09.12  

  • 4重鎖核酸選択的キノン-シアニン色素リガンドによる細胞死の光誘導

    村元順哉, 坂本隆

    第34回バイオ・高分子シンポジウム  2024.08.02  

  • 3脚型キノンーシアニン色素への光照射により生成する活性酸素によるアポトーシス誘導

    村元順哉, 安原優, 坂本隆

    日本ケミカルバイオロジー学会第18回年会  2024.05.28  

  • Increased sensitivity of HCR-based nucleic acid detection using the concentration effect of microbeads

    Yu Yasuhara, Takashi Sakamoto

    日本化学会第103春季年会  2024.03.19  

  • 核酸4重鎖と2重鎖を見分ける近赤外蛍光プローブ

    坂本隆  [Invited]

    分子夾雑に挑む化学@和歌山  2023.12.01  

  • Fluorescence imaging of nucleic acid quadruplex structure using D3A-type quinone-cyanine fluorescent dyes

    Takashi Sakamoto, Yuka Muraoka, Zehui Yu, Mayuko Kawatake

    The 50th International Symposium on Nucleic Acids Chemistry (ISNAC2023)  2023.11.02  

  • D3A型キノン-シアニン蛍光色素の核酸認識に与えるアクセプター構造の影響

    坂本隆, 村岡優香, 于ゾウ慧, 川竹万柚子

    第17回バイオ関連化学シンポジウム  2023.09.09  

  • D3A 型キノン-シアニン蛍光色素による核酸イメージング

    坂本隆, 村岡優香, 于ゾウ慧, 川竹万柚子

    第33回バイオ・高分子シンポジウム  2023.07.28  

  • DNA2重鎖・4重鎖二色蛍光スイッチオンプローブの結合選択性の制御

    村岡 優香, 安原 優, 岩井 まり奈, 坂本 隆

    日本化学会第103春季年会  2023.03.22  

  • がんの光線力学療法を志向した三脚型キノン−シアニン色素の活性酸素生成能の評価

    村元順哉, 安原優, 坂本隆

    日本化学会第103春季年会  2023.03.22  

  • Controlling the fluorescence response of tripodal quinone-cyanine dyes upon nucleic acid binding

    Takashi Sakamoto, Yuka Muraoka, Yu Yasuhara, Marina Iwai

    The 49th International Symposium on Nucleic Acids Chemistry (ISNAC2022)  2022.11.03  

  • がんの光線力学療法を指向した3脚型キノンーシアニン色素の光増感活性の評価

    村元順哉, 安原優, 坂本隆

    第16回バイオ関連化学シンポジウム  2022.09.10  

  • 3脚型キノン-シアニン蛍光色素の核酸構造認識を制御するための化学修飾

    村岡優香, 安原優, 岩井まり奈, 坂本隆

    第16回バイオ関連化学シンポジウム  2022.09.10  

  • 生細胞内局所粘度イメージングを目指した3脚型キノンーシアニン近赤外蛍光色素の粘度応答性能の制御

    坂本隆, 岩井まり奈

    日本ケミカルバイオロジー学会第16回年会  2022.05.31  

  • 3脚型キノン-シアニン色素誘導体の4重鎖および2重鎖DNAに対する蛍光応答

    村岡優香, 安原優, 岩井まり奈, 坂本隆

    日本ケミカルバイオロジー学会第16回年会  2022.05.31  

  • 蛍光特性の光制御が可能なフォトクロミックDNA蛍光イメージングプローブの開発

    三輪好輝, 坂本隆

    日本化学会第102春季年会  2022.03.24  

  • 種々のベンゾチアゾリウムカチオン誘導体を含む3脚型キノン-シアニン蛍光色素の核酸に対する蛍光応答

    村岡優香, 坂本隆

    日本化学会第102春季年会  2022.03.23  

  • 膜透過性官能基を導入した三脚型キノン−シアニン蛍光色素の合成

    大谷悠人, 坂本隆

    日本化学会第102春季年会  2022.03.23  

  • 核酸構造の色分けイメージングを可能とする蛍光プローブの開発

    坂本隆  [Invited]

    第17回若手技術者交流会(主催:和歌山県化学技術者協会)  2022.03.03  

  • A dual fluorescence turn-on probe for simultaneous imaging of double-stranded DNA and G4 DNA

    Takashi Sakamoto, Zehui Yu, Yuto Otani

    The 48th International Symposium on Nucleic Acids Chemistry (ISNAC2021)  2021.11.12  

  • A dual-color fluorescence switch-on probe for imaging the dynamics of guanine quadruplexes in cells

    Takashi Sakamoto  [Invited]

    FIBER Webinar Universe in Nucleic Acids Chemistry 4  2021.11.02  

  • DNA構造に選択的な2色蛍光スイッチオンプローブを用いた4重鎖構造形成のリアルタイム追跡

    坂本隆, 于ゾウ慧, 大谷悠人

    第15回バイオ関連化学シンポジウム  2021.09.10  

  • 2重鎖と4重鎖を色分けして検出できる蛍光プローブを用いた細胞内DNAイメージング

    坂本隆, 于ゾウ慧, 大谷悠人

    第31回バイオ・高分子シンポジウム  2021.06.25  

  • A Tripodal Quinone-Cyanine Fluorescent Dye for G4 Nucleic Acids Imaging in Cells

    Takashi Sakamoto, Zehui Yu, Yuto Otan

    日本ケミカルバイオロジー学会第15回年会  2021.06.23  

  • 多点認識によるねじれ型分子内電荷移動制御に基づく糖検出蛍光プローブの開発

    佐藤頌, 坂本隆

    日本化学会第101春季年会  2021.03.19  

  • 三脚型キノン-シアニン蛍光プローブによる2重鎖及び4重鎖DNAの同時検出

    坂本隆, 于ゾウ慧, 大谷悠人

    日本化学会第101春季年会  2021.03.19  

  • ANDゲート型近赤外蛍光分子プローブを用いた細胞核内脱リン酸化酵素活性イメージング

    添田嵩章, 坂本隆

    日本化学会第101春季年会  2021.03.19  

  • 低分子蛍光プローブを用いたDNA2重鎖形成連鎖反応のラベルフリー検出系の構築

    山田陸斗, 森山貴登, 坂本隆

    第30回バイオ・高分子シンポジウム  2020.07.31  

  • 核内の脱リン酸化酵素活性に応答する近赤外蛍光分子プローブの開発

    添田嵩章, 坂本隆

    日本化学会第100春季年会  2020.03.24  

  • 改変型OPA反応と19F NMRを利用したアミノ酸の同時一斉分析

    坂本隆, QIU Zhiyong, 稲垣美侑, 藤本健造

    日本化学会第100春季年会  2020.03.24  

  • グルーブバインダーの2重鎖DNA配列選択的な相互作用を利用したハイブリダイゼーション連鎖反応のラベルフリー検出

    坂本隆, 山田陸斗

    日本化学会第100春季年会  2020.03.22  

  • Label-free in situ monitoring of the DNA hybridization chain reaction by using sequence-selective minor-groove-binding fluorophores

    Takashi Sakamoto, Rikuto Yamada

    The 46th International Symposium on Nucleic Acids Chemistry (ISNAC2019)  2019.10.29  

  • 脱リン酸化酵素に応答するANDゲート型DNA検出蛍光プローブの開発

    添田嵩章, 坂本隆

    第13回バイオ関連化学シンポジウム  2019.09.05  

  • DNAマイナーグルーブ結合型蛍光色素を用いたハイブリダイゼーション連鎖反応のラベルフリー検出

    山田陸斗, 坂本隆

    日本化学会第99春季年会  2019.03.18  

  • ヘキスト系色素の非標準構造DNAに対する光反応の検討

    三輪好輝, 瀬戸上慧, 坂本隆

    日本化学会第99春季年会  2019.03.17  

  • 19F核磁気共鳴を用いた生体分子の同時一斉計測

    坂本隆  [Invited]

    2018ハロゲン利用ミニシンポジウム  2018.12.07  

  • 細胞内ホスファターゼ活性イメージングを指向したANDゲート型蛍光プローブの開発

    坂本隆

    第28回バイオ・高分子シンポジウム  2018.07.26  

  • Development of fluorescent molecular probe for imaging tyrosine phosphatase activity in cell nuclei

    Takashi Sakamoto

    日本化学会第98春季年会  2018.03.21  

  • まとめてイッキに分析!〜生体分子の同時一斉解析技術〜

    坂本隆  [Invited]

    メディカルジャパン2018大阪 「研究成果企業化促進セミナー」  2018.02.21  

  • 19F NMR を用いたアミノ酸同時一斉解析法の開発

    坂本隆, QIU Zhiyong, 藤本健造

    第11回バイオ関連化学シンポジウム  2017.09.08  

  • 19F NMR を用いた核酸等生体分子の同時一斉計測技術の開発

    坂本隆, 長谷川大策, QIU Zhiyong, 藤本健造

    第27回バイオ・高分子シンポジウム(2017.7.27)  2017.07.27  

  • 二本鎖 DNA 中での3ーシアノビニルカルバゾールを用いた光化学的デアミネーションにおけるシトシンの対合塩基の影響

    SETHI Siddhant, 大江美成子, 坂本隆, 中村重孝, 藤本健造

    日本薬学会第137年会  2017.03.25  

  • Development of RNA FISH using photoresponsive molecular beacon probe

    Chinami Kano, Kei Toyosato, Takashi Sakamoto, Shigetaka Nakamura, Kenzo Fujimoto

    日本化学会第97春季年会  2017.03.17  

  • Development of novel molecular devices capable of photo-cross-linking with DNA having specific structures

    Takashi Sakamoto, Kenzo Fujimoto

    日本化学会第97春季年会  2017.03.17  

  • Biomolecule simultaneous analysis by using 19F NMR

    Zhiyong Qiu, Takashi Sakamoto, Kenzo Fujimoto

    日本化学会第97春季年会  2017.03.16  

  • 光化学的なRNA架橋反応を用いた新規FISH 法の開発

    中村重孝, 豊里慧, 狩野千波, 坂本隆, 藤本健造

    第39回日本分子生物学会年会  2016.11.30  

  • Evaluation of role of hydrogen bonding in deamination of 3-cyanovinylcarbazole mediated photo-cross-linked cytosine in DNA duplex

    Siddhant Sethi, Minako Ooe, Takashi Sakamoto, Kenzo Fujimoto

    平成28年度北陸地区講演会と研究発表会  2016.11.25  

  • 光架橋反応を用いた新規 RNA FISH 法の開発

    狩野千波, 豊里慧, 中村重孝, 坂本隆, 藤本健造

    平成28年度北陸地区講演会と研究発表会  2016.11.25  

  • 19F NMRを用いた遊離アミノ酸の同時一斉分析

    QIU Zhiyong, 坂本隆, 藤本健造

    平成28年度北陸地区講演会と研究発表会  2016.11.25  

  • Effects of hydrogen bonding on the cytosine deamination in photo-cross-linked DNA duplex

    Siddhant Sethi, Takashi Sakamoto, Kenzo Fujimoto

    The 43rd International Symposium on Nucleic Acids Chemistry (ISNAC2016)  2016.09.27  

  • RNA FISH using 3-cyanovinylcarbazole-modified ODNs as photo-cross-linking probe

    Chinami Kano, Kei Toyosato, Shigetaka Nakamura, Takashi Sakamoto, Kenzo Fujimoto

    The 43rd International Symposium on Nucleic Acids Chemistry (ISNAC2016)  2016.09.27  

  • Disassembly-driven signal turn-on probe for multimodal detection of DNAs using 19F NMR and fluorescence

    Takashi Sakamoto, Daisaku Hasegawa, Kenzo Fujimoto

    The 43rd International Symposium on Nucleic Acids Chemistry (ISNAC2016)  2016.09.27  

  • 蛍光・19F NMRデュアルモードDNA検出を可能とするOFF/ON型分子プローブの開発

    坂本隆, 長谷川大策, 藤本健造

    第10 回バイオ関連化学シンポジウム  2016.09.07  

  • シアノビニルカルバゾールによって光架橋された DNA 上シトシンの脱アミノ化における水素結合の影響

    SETHI Siddhant, 坂本隆, 藤本健造

    第10 回バイオ関連化学シンポジウム  2016.09.07  

  • 高速光架橋能を有する蛍光プローブを用いたRNA FISH

    狩野 千波, 豊里慧, 中村重孝, 坂本隆, 藤本健造

    第10 回バイオ関連化学シンポジウム  2016.09.07  

  • 改変型OPA反応を利用した迅速アミノ酸分析法の開発

    QIU Zhiyong, 坂本隆, 藤本健造

    第10 回バイオ関連化学シンポジウム  2016.09.07  

  • 分子ロボのための核酸光操作ツールの開発

    坂本隆  [Invited]

    第4回分子ロボ若手会  2016.08.20  

  • フッ素標識ヘキストプローブを用いた 19F NMR による DNA 検出

    坂本隆, 長谷川大策, 藤本健造

    第26 回バイオ・高分子シンポジウム  2016.07.29  

  • 19F NMRを用いたDNA1塩基多型の同時一斉解析

    坂本隆, 長谷川大策, 藤本健造

    日本化学会第96春季年会  2016.03.27  

  • Development of molecular probes for 19F NMR/fluorescence bimodal detection of DNAs in living cells

    Takashi Sakamoto, Daisaku Hasegawa, Kenzo Fujimoto

    PACIFICHEM 2015  2015.12.15  

  • 19F NMR-based analysis of the local sequence and structure of DNAs using fluorine-modified bisbenzimide derivative

    Daisaku Hasegawa, Takashi Sakamoto, Kenzo Fujimoto

    PACIFICHEM 2015  2015.12.15  

  • Development of a novel fluorescence in situ hybridization method using photoreactive oligonuculeotide as a fluorescent probe

    Kei Toyosato, Takashi Sakamoto, Kenzo Fujimoto

    PACIFICHEM 2015  2015.12.15  

  • Photochemical site-directed mutagenesis using 3-cyanovinylcarbazole modified oligodeoxynucleotide

    Siddhant Sethi, Minako Ooe, Takashi Sakamoto, Kenzo Fujimoto

    The 42nd International Symposium on Nucleic Acids Chemistry (ISNAC2015)  2015.09.23  

  • Fluorine-modified bisbenzimide derivative as a molecular probe for simultaneous detection of DNAs by 19F NMR

    Takashi Sakamoto, Daisaku Hasegawa, Kenzo Fujimoto

    The 42nd International Symposium on Nucleic Acids Chemistry (ISNAC2015)  2015.09.23  

  • 異なる核酸の同時検出を可能にする新規核酸検出プローブの開発

    坂本隆, 長谷川大策, 藤本健造

    第9回バイオ関連化学シンポジウム  2015.09.11  

  • 19F核磁気共鳴による核酸検出を可能とする新規分子プローブの開発

    坂本隆, 長谷川大策, 藤本健造

    第 25 回バイオ・高分子シンポジウム  2015.07.24  

  • 蛍光および19F核磁気共鳴によるDNA検出を可能にする新たな分子プローブの開発

    坂本隆, 長谷川大策, 藤本健造

    日本ケミカルバイオロジー学会第10回年会  2015.06.12  

  • Hoechst 33258骨格を有するDNA検出19F核磁気共鳴プローブの開発

    坂本隆, 長谷川大策, 藤本健造

    日本化学会第95春季年会  2015.03.26  

  • Effect of duplex stability on the cytosine deamination using DNA photo-cross-linking reaction

    Minako Oe, Takashi Sakamoto, Kenzo Fujimoto

    The 41st International Symposium on Nucleic Acids Chemistry (ISNAC2014)  2014.11.05  

  • To probe human telomere RNA G-quadruplex structure by 19F NMR

    Hong-Liang Bao, Takumi Ishizuka, Takashi Sakamoto, Kenzo Fujimoto, Yan Xu

    The 41st International Symposium on Nucleic Acids Chemistry (ISNAC2014)  2014.11.05  

  • Short oligonucleotide having 5-fluoro and 5-iodouracil acts as an anticancer prodrug

    Mirei Furusawa, Yu-ki Takematsu, Atsuo Shigeno, Takashi Sakamoto, Kenzo Fujimoto

    The 41st International Symposium on Nucleic Acids Chemistry (ISNAC2014)  2014.11.05  

  • 核磁気共鳴法に基づく新たな生体分子検出・イメージングプローブの開発

    坂本隆  [Invited]

    平成26年度 第1回テニュアトラック制度シンポジウム −生命システムに挑む機能分子の化学–  2014.10.17  

  • 高速光架橋性アンチセンス核酸による細胞内遺伝子発現の光制御

    坂本隆, 滋野敦夫, 大滝優一, 藤本 健造

    第8回バイオ関連化学シンポジウム  2014.09.12  

  • 高速光架橋性アンチセンス核酸による細胞内遺伝子発現制御

    坂本隆, 滋野敦夫, 大滝優一, 藤本 健造

    アンチセンス・遺伝子・デリバリーシンポジウム2014  2014.09.08  

  • 光架橋性オリゴヌクレオチドによる細胞内遺伝子発現制御法の開発

    坂本隆, 滋野敦夫, 大滝優一, 藤本健造

    第36回日本光医学・光生物学会  2014.07.25  

  • 高速光架橋性オリゴヌクレオチドによる細胞内遺伝子発現制御

    坂本隆, 滋野敦夫, 大滝優一, 藤本健造

    日本ケミカルバイオロジー学会第9回年会  2014.06.12  

▼display all

Patents

  • アミノ酸分析法

    Patent no: 6725144

    Date registered: 2020.06.29 

    Date applied: 2016.09.06 ( 特願2016-173792 )   Publication date: 2018.03.15 ( 特開2018-39747 )  

    Inventor(s)/Creator(s): 坂本隆  Applicant: 国立大学法人和歌山大学

  • 核酸検出方法、化合物、及び蛍光プローブ

    米国

    Date applied: 2021.11.30 ( 18/266187 )  

    Inventor(s)/Creator(s): 坂本 隆  Applicant: 国立大学法人和歌山大学

  • 核酸検出方法、化合物、及び蛍光プローブ

    欧州

    Date applied: 2021.11.30 ( 21903252.1 )  

    Inventor(s)/Creator(s): 坂本 隆  Applicant: 国立大学法人和歌山大学

  • 核酸検出方法、化合物、及び蛍光プローブ

    Date applied: 2020.12.11 ( 特願2020-206368 )   Public disclosure date: 2022.06.16 ( WO2022/124148 )

    Inventor(s)/Creator(s): 坂本 隆  Applicant: 国立大学法人和歌山大学

Research Exchange

  • ライフサイエンス分野 新技術説明会

    2018.01
     
  • 第26回わかやまテクノビジネスフェア

    2017.11
     
  • 大阪府立大学・和歌山大学工学研究シーズ合同発表会

    2017.10
     

KAKENHI

  • 三脚型キノン-シアニン色素の特徴を活かした4重鎖核酸イメージング蛍光色素の開発

    2021.04
    -
    2024.03
     

    Grant-in-Aid for Scientific Research(C)  Principal investigator

  • 超高速光クロスリンクを用いたゲノム操作法の開発

    2017.04
    -
    2020.03
     

    Grant-in-Aid for Scientific Research(B)  Co-investigator

  • DNA捕捉・放出能力をもつ多機能性ペプチド・ゲル・リポソームの創出

    2015.04
    -
    2017.03
     

    Grant-in-Aid for Scientific Research on Innovative Areas(Research in a Proposed Research Area)  Principal investigator

  • レシオ変化型核磁気共鳴プローブによる核酸の同時並列的検出法の開発

    2015.04
    -
    2017.03
     

    Grant-in-Aid for Young Scientists(B)  Principal investigator

  • 光架橋反応を用いた19F-NMRによる核酸類検出法の開発

    2014.04
    -
    2017.03
     

    Grant-in-Aid for Scientific Research(B)  Co-investigator

Instructor for open lecture, peer review for academic journal, media appearances, etc.

  • 投稿論文の査読

    2024.06

    Bioorganic Medicinal Chemistry Letters

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2024.05

    European Journal of Organic Chemistry

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2023.12

    Theranostics

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2022.12

    Analytical Chemistry, ACS

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 「第2回Challenge万博」での講演

    2022.11.24

    関西イノベーションイニシアティブ(KSII)、三井住友銀行

     View Details

    蛍光プローブ

    「病気の原因になる特殊なDNA構造を見つけ出す蛍光色素」に関する講演

  • 和歌山県化学技術者協会講演会での講演

    2022.11.14

    和歌山県化学技術者協会

     View Details

    蛍光プローブ

    「細胞内の核酸構造を色分けできる蛍光色素の開発」に関する講演

  • MOBIO産学連携オフィス テーマ別合同シーズ発表会 「万博」での講演

    2022.10.18

    公益財団法人大阪産業局

     View Details

    蛍光プローブ

    『核酸構造の色分けイメージングを可能とするカメレオンみたいな蛍光プローブ』に関する講演

  • 関西バイオビジネスマッチング2022での講演

    2022.10.03
    -
    2022.11.18

    バイオコミュニティ関西(BiocK)・NPO法人近畿バイオインダストリー振興会議

     View Details

    蛍光プローブ

    「細胞内4重鎖DNAを簡単に見るための蛍光試薬」に関する講演

  • 投稿論文の査読

    2022.10

    Analytical Chemistry, ACS

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2022.09

    Organic & Biomolecular Chemistry, RSC

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2022.05

    Chemistry and Biodiversity

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2022.04

    Bioorganic Chemistry

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 第17回若手技術者交流会での講演

    2022.03.03

    和歌山県化学技術者協会

     View Details

    蛍光プローブ

    「核酸構造の色分けイメージングを可能とする蛍光プローブの開発」に関する講演

  • 投稿論文の査読

    2022.03

    Chemistry and Biodiversity, Wiley-VCH

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2021.11

    Magnetic Resonance in Chemistry, Wiley-VCH

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2021.10

    Advanced Functional Materials, Wiley-VCH

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2021.09

    ACSOmega, American Chemical Society

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • イノベーションジャパン 2021 ~大学見本市 Online での講演

    2021.08.23
    -
    2021.09.17

    科学技術振興機構

     View Details

    蛍光プローブ

    「DNA2重鎖と4重鎖を蛍光色で見分ける2色蛍光スイッチオンプローブ色素」に関する講演

  • 投稿論文の査読

    2021.08

    Organic & Biomolecular Chemistry, Royal Society of Chemistry

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 夢ナビライブでの高校生向けのオンライン講演

    2021.04
    -
    2021.07

    株式会社 フロムページ

     View Details

    核酸研究

    オンライン講演

  • 投稿論文の査読

    2021.04

    Analytica Chimica Acta, Elsevier

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2021.04

    Organic & Biomolecular Chemistry, Royal Society of Chemistry

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 国立大学56工学系学部おもしろ科学実験室への記事の提供

    2021.03.19

    国立大学56工学系学部長会議

     View Details

    抗菌作用

    国立大学56工学系学部おもしろ科学実験室への記事の提供

  • 投稿論文の査読

    2020.04
    -
    2021.01

    Biomaterials Science, Royal Society of Chemistry

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2020.04
    -
    2020.09

    ChemBioChem, Wiley-VCH

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2020.04
    -
    2020.08

    Organic & Biomolecular Chemistry, Royal Society of Chemistry

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 投稿論文の査読

    2020.04
    -
    2020.05

    ChemBioChem, Wiley-VCH

     View Details

    学術雑誌等の編集委員・査読・審査員等

    投稿論文の査読

  • 講師

    2018.02.21

    「メディカルジャパン2018大阪」研究成果企業化促進セミナー

     View Details

    講演講師等

    講師,任期:2018年2月~

  • 研究者交流会(精密物質学科 卒業研究発表会 午前の部終了後)

    2016.04

    和歌山大学システム工学部精密物質学科

     View Details

    公開講座・講演会の企画・講師等

    講演(話題提供),日付:2016.2.28

▼display all

Committee member history in academic associations, government agencies, municipalities, etc.

  • 口頭発表 座長

    2021.03.19
     

    日本化学会第101春季年会(日本化学会)

     View Details

    学協会、政府、自治体等の公的委員

    口頭発表の座長

Other Social Activities

  • 「第2回Challenge万博」での講演

    2022.11.24

    関西イノベーションイニシアティブ(KSII)、三井住友銀行

     View Details

    蛍光プローブ

    「病気の原因になる特殊なDNA構造を見つけ出す蛍光色素」に関する講演
    産学連携イノベーションセンターの依頼に基づき実施
    社会活動・項目群I にも記載

  • MOBIO産学連携オフィス テーマ別合同シーズ発表会 「万博」での講演

    2022.10.18

    公益財団法人大阪産業局

     View Details

    蛍光プローブ

    『核酸構造の色分けイメージングを可能とするカメレオンみたいな蛍光プローブ』に関する講演
    産学連携イノベーションセンターの依頼に基づき実施
    社会活動・項目群I にも記載

  • 関西バイオビジネスマッチング2022での講演

    2022.10.03
    -
    2022.11.18

    バイオコミュニティ関西(BiocK)・NPO法人近畿バイオインダストリー振興会議

     View Details

    蛍光プローブ

    「細胞内4重鎖DNAを簡単に見るための蛍光試薬」に関する講演
    産学連携イノベーションセンターの依頼に基づき実施
    社会活動・項目群I にも記載

  • イノベーション・ジャパン2021~大学見本市Online

    2021.08.23
    -
    2021.09.17

    その他

     View Details

    社会との連携を推進する活動

    オンライン発表

  • 和歌山県化学技術者協会 講演会

    2019.04
    -
    2020.03

    その他

     View Details

    社会との連携を推進する活動

    ポスター発表