Published Papers - SAKAMOTO Takashi

Bispyrene-Conjugated 2′-O-Methyloligonucleotide as a Highly Specific RNA-Recognition Probe

Mahara, Atsushi, Iwase, Reiko, Sakamoto, Takashi, Yamana, Kazushige, Yamaoka, Tetsuji, Murakami, Akira

Angewandte Chemie ( WILEY-VCH Verlag )  114 ( 19 ) 3800 - 3802   2002  [Refereed]

DOI

Study on structure of ribosomal RNA by time-resolved luminescence anisotropy analysis.

T. Sakamoto, A. Mahara, R. Iwase, T. Yamaoka, A. Murakami （Part： Lead author )

Nucleic acids research. Supplement (2001)   1 ( 1 ) 143 - 144   2001  [Refereed]

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Evaluation of the folded structure of E.coli 16S-rRNA was performed using 5'-Ru(II) complex labeled oligodeoxyribonucleotide (Ru-probe) as the probe. This probe could evaluate the dynamic manner of the binding site of Ru-probe by using the time-resolved luminescence anisotropy technique. It was found that the rotational manner of Ru-probe in the presence of 16S-rRNA was markedly dependent on the oligonucleotide sequence, indicating that the microenvironments around the Ru-probes were largely different.

DOI