論文 - 坂本　隆

Bispyrene-Conjugated 2′-O-Methyloligonucleotide as a Highly Specific RNA-Recognition Probe

Mahara Atsushi, Iwase Reiko, Sakamoto Takashi, Yamana Kazushige, Yamaoka Tetsuji, Murakami Akira

Angewandte Chemie ( WILEY-VCH Verlag )  114 ( 19 ) 3800 - 3802   2002年  [査読有り]

DOI

Study on structure of ribosomal RNA by time-resolved luminescence anisotropy analysis.

T. Sakamoto, A. Mahara, R. Iwase, T. Yamaoka, A. Murakami (担当区分： 筆頭著者 )

Nucleic acids research. Supplement (2001)   1 ( 1 ) 143 - 144   2001年  [査読有り]

　概要を見る

Evaluation of the folded structure of E.coli 16S-rRNA was performed using 5'-Ru(II) complex labeled oligodeoxyribonucleotide (Ru-probe) as the probe. This probe could evaluate the dynamic manner of the binding site of Ru-probe by using the time-resolved luminescence anisotropy technique. It was found that the rotational manner of Ru-probe in the presence of 16S-rRNA was markedly dependent on the oligonucleotide sequence, indicating that the microenvironments around the Ru-probes were largely different.

DOI