Updated on 2024/06/18

写真a

 
KAJIMURA MAKIKO
 
Name of department
Faculty of Education, Science Education
Job title
Associate Professor
Mail Address
E-mail address
External link

Degree

  • 博士(理学)

Association Memberships

  • -
    Now

    日本比較生理生化学会

Research Areas

  • Life sciences / Animals: biochemistry, physiology, behavioral science

Classes (including Experimental Classes, Seminars, Graduation Thesis Guidance, Graduation Research, and Topical Research)

  • 2023   Teaching Methods in Integrated Studies   Specialized Subjects

  • 2023   Science Education Contents   Specialized Subjects

  • 2023   seminar of animal physiology   Specialized Subjects

  • 2023   Seminar of Scientific Education   Specialized Subjects

  • 2023   Researching Science Teaching Materials   Specialized Subjects

  • 2023   Young Children and Environment   Specialized Subjects

  • 2023   Experiment of Biology   Specialized Subjects

  • 2023   Life   Specialized Subjects

  • 2023   Science education in juinor high school C   Specialized Subjects

  • 2023   Environmental physiology   Specialized Subjects

  • 2023   Biology A   Specialized Subjects

  • 2022   Teaching Methods in Integrated Studies   Specialized Subjects

  • 2022   Experiment of Biology   Specialized Subjects

  • 2022   Science Education Contents   Specialized Subjects

  • 2022   seminar of animal physiology   Specialized Subjects

  • 2022   Seminar of Scientific Education   Specialized Subjects

  • 2022   Life   Specialized Subjects

  • 2022   Science education in juinor high school C   Specialized Subjects

  • 2022   Environmental physiology   Specialized Subjects

  • 2022   Biology A   Specialized Subjects

  • 2021   Seminar of Scientific Education   Specialized Subjects

  • 2021   Science Education Contents   Specialized Subjects

  • 2021   Researching Science Teaching Materials   Specialized Subjects

  • 2021   Teaching Methods in Integrated Studies   Specialized Subjects

  • 2021   Experiment of Biology   Specialized Subjects

  • 2021   Experiment of Biology   Specialized Subjects

  • 2021   seminar of animal physiology   Specialized Subjects

  • 2021   Science education in juinor high school C   Specialized Subjects

  • 2021   Environmental physiology   Specialized Subjects

  • 2021   Biology A   Specialized Subjects

  • 2021   Life   Specialized Subjects

  • 2020   Experiment of Biology   Specialized Subjects

  • 2020   Science Education Contents   Specialized Subjects

  • 2020   seminar of animal physiology   Specialized Subjects

  • 2020   Science education in juinor high school C   Specialized Subjects

  • 2020   Environmental physiology   Specialized Subjects

  • 2020   Biology A   Specialized Subjects

  • 2019   Experiment of Biology   Specialized Subjects

  • 2019   Experiment of Biology   Specialized Subjects

  • 2019   Science Education Contents   Specialized Subjects

  • 2019   Seminar of Scientific Education   Specialized Subjects

  • 2019   Seminar of Scientific Education   Specialized Subjects

  • 2019   Science education in juinor high school C   Specialized Subjects

  • 2018   Science Education Contents   Specialized Subjects

  • 2018   Seminar of Scientific Education   Specialized Subjects

  • 2018   Life   Specialized Subjects

  • 2018   Science education in juinor high school C   Specialized Subjects

  • 2018   BioliteracyⅡ   Specialized Subjects

  • 2018   BioliteracyⅠ   Specialized Subjects

  • 2017   Science Education Contents   Specialized Subjects

  • 2017   Science education in juinor high school C   Specialized Subjects

  • 2017   BioliteracyⅡ   Specialized Subjects

  • 2017   BioliteracyⅠ   Specialized Subjects

  • 2016   Science education in juinor high school C   Specialized Subjects

  • 2016   BioliteracyⅡ   Specialized Subjects

  • 2016   BioliteracyⅠ   Specialized Subjects

  • 2014   Seminar on Environmental Education(animal physiology)   Specialized Subjects

  • 2014   seminar of animal physiology   Specialized Subjects

  • 2014   Marine Practice of Biology   Specialized Subjects

  • 2014   Life   Specialized Subjects

  • 2014   Science education in juinor high school C   Specialized Subjects

  • 2014   Experiment on Biological Science (Biology)   Specialized Subjects

  • 2014   BioliteracyⅡ   Specialized Subjects

  • 2014   BioliteracyⅠ   Specialized Subjects

  • 2014   Environmental physiology   Specialized Subjects

  • 2014   Science   Specialized Subjects

  • 2014   Elementary Experiment of Biology B   Specialized Subjects

  • 2014   Elementary Experiment of Biology A   Specialized Subjects

  • 2014   Biology A   Specialized Subjects

  • 2013   NA   Specialized Subjects

  • 2013   Seminar on Environmental Education(animal physiology)   Specialized Subjects

  • 2013   seminar of animal physiology   Specialized Subjects

  • 2013   Marine Practice of Biology   Specialized Subjects

  • 2013   Science education in juinor high school C   Specialized Subjects

  • 2013   Experiment on Biological Science (Biology)   Specialized Subjects

  • 2013   BioliteracyⅡ   Specialized Subjects

  • 2013   BioliteracyⅠ   Specialized Subjects

  • 2013   Environmental physiology   Specialized Subjects

  • 2013   Evolution of Earth's Environment   Specialized Subjects

  • 2013   Elementary Experiment of Biology B   Specialized Subjects

  • 2013   Elementary Experiment of Biology A   Specialized Subjects

  • 2013   Biology A   Specialized Subjects

  • 2012   NA   Specialized Subjects

  • 2012   seminar of animal physiology   Specialized Subjects

  • 2012   Seminar on Environmental Education(animal physiology)   Specialized Subjects

  • 2012   Practical Study of Manufacturing and Science   Specialized Subjects

  • 2012   Marine Practice of Biology   Specialized Subjects

  • 2012   Experiment on Biological Science (Biology)   Specialized Subjects

  • 2012   Elementary Experiment of Biology A   Specialized Subjects

  • 2012   Biology A   Specialized Subjects

  • 2012   Environmental physiology   Specialized Subjects

  • 2012   BioliteracyⅠ   Specialized Subjects

  • 2012   Science education in juinor high school C   Specialized Subjects

  • 2012   Elementary Experiment of Biology B   Specialized Subjects

  • 2012   Adaptation and Environment   Specialized Subjects

  • 2012   BioliteracyⅡ   Specialized Subjects

  • 2011   Biology A   Specialized Subjects

  • 2011   Practical Study of Manufacturing and Science   Specialized Subjects

  • 2011   Seminar on Environmental Education(animal physiology)   Specialized Subjects

  • 2011   Marine Practice of Biology   Specialized Subjects

  • 2011   Seminar on Natural Environments (Earth)   Specialized Subjects

  • 2011   Experiment on Biological Science (Biology)   Specialized Subjects

  • 2011   Elementary Experiment of Biology B   Specialized Subjects

  • 2011   Elementary Experiment of Biology A   Specialized Subjects

  • 2011   Science education in juinor high school C   Specialized Subjects

  • 2011   NA   Specialized Subjects

  • 2011   Adaptation and Environment   Specialized Subjects

  • 2011   Environmental physiology   Specialized Subjects

  • 2011   Evolution of Earth's Environment   Specialized Subjects

  • 2011   Biology A   Specialized Subjects

  • 2011   Seminar on Natural Environments (Earth)   Specialized Subjects

  • 2011   Seminar on Environmental Education(Nature)   Specialized Subjects

  • 2011   Marine Practice of Biology   Specialized Subjects

  • 2011   Practical Study of Manufacturing and Science   Specialized Subjects

  • 2011   Seminar on Biological Science   Specialized Subjects

  • 2011   Science education in juinor high school C   Specialized Subjects

  • 2011   BioliteracyⅡ   Specialized Subjects

  • 2011   NA   Specialized Subjects

  • 2011   Adaptation and Environment   Specialized Subjects

  • 2011   Evolution of Earth's Environment   Specialized Subjects

  • 2011   Elementary Experiment of Biology B   Specialized Subjects

  • 2011   Elementary Experiment of Biology A   Specialized Subjects

  • 2010   Practical Study of Manufacturing and Science   Specialized Subjects

  • 2010   Seminar on Environmental Education(Nature)   Specialized Subjects

  • 2010   Marine Practice of Biology   Specialized Subjects

  • 2010   Seminar on Natural Environments (Earth)   Specialized Subjects

  • 2010   Science education in juinor high school C   Specialized Subjects

  • 2010   Seminar on Biological Science   Specialized Subjects

  • 2010   Experiment on Biological Science (Biology)   Specialized Subjects

  • 2010   BioliteracyⅡ   Specialized Subjects

  • 2010   NA   Specialized Subjects

  • 2010   Adaptation and Environment   Specialized Subjects

  • 2010   Evolution of Earth's Environment   Specialized Subjects

  • 2010   Elementary Experiment of Biology B   Specialized Subjects

  • 2010   Elementary Experiment of Biology A   Specialized Subjects

  • 2010   Biology A   Specialized Subjects

  • 2009   Practical Study of Manufacturing and Science   Specialized Subjects

  • 2009   Marine Practice of Biology   Specialized Subjects

  • 2009   Experiment on Biological Science (Biology)   Specialized Subjects

  • 2009   Seminar on Biological Science   Specialized Subjects

  • 2009   Elementary Experiment of Biology B   Specialized Subjects

  • 2009   Elementary Experiment of Biology A   Specialized Subjects

  • 2009   BioliteracyⅡ   Specialized Subjects

  • 2009   BioliteracyⅠ   Specialized Subjects

  • 2009   Seminar on Natural Environments (Earth)   Specialized Subjects

  • 2009   Science education in juinor high school C   Specialized Subjects

  • 2009   Evolution of Earth's Environment   Specialized Subjects

  • 2009   Biology A   Specialized Subjects

  • 2009   Adaptation and Environment   Specialized Subjects

  • 2009   Environmental physiology   Specialized Subjects

▼display all

Satellite Courses

  • 2014   NA   Liberal Arts and Sciences Subjects

  • 2013   NA   Liberal Arts and Sciences Subjects

  • 2012   NA   Liberal Arts and Sciences Subjects

  • 2011   NA   Liberal Arts and Sciences Subjects

  • 2010   NA   Liberal Arts and Sciences Subjects

Classes

  • 2021   Development of Inquisitive Teaching Materials - Rice  

  • 2021   Seminar of animal physiology   Master's Course

  • 2020   Development of Inquisitive Teaching Materials - Rice   Master's Course

  • 2020   Seminar of animal physiology   Master's Course

  • 2019   Development of Inquisitive Teaching Materials - Rice   Master's Course

  • 2019   Seminar of animal physiology   Master's Course

  • 2019   Seminar of animal physiology   Master's Course

  • 2018   Seminar of animal physiology   Master's Course

  • 2018   Seminar of animal physiology   Master's Course

  • 2018   Animal physiology advanced   Master's Course

  • 2018   Animal physiology advanced   Master's Course

  • 2017   Seminar of animal physiology   Master's Course

  • 2017   Seminar of animal physiology   Master's Course

  • 2017   Animal physiology advanced   Master's Course

  • 2017   Animal physiology advanced   Master's Course

  • 2016   Animal physiology advanced   Master's Course

  • 2016   Animal physiology advanced   Master's Course

  • 2014   Seminar of animal physiology  

  • 2014   Seminar of animal physiology  

  • 2014   Animal physiology advanced  

  • 2014   Animal physiology advanced  

  • 2013   Seminar of animal physiology  

  • 2013   Seminar of animal physiology  

  • 2013   Animal physiology advanced  

  • 2013   Animal physiology advanced  

  • 2012   Seminar of animal physiology  

  • 2012   Seminar of animal physiology  

  • 2012   Animal physiology advanced  

  • 2012   Animal physiology advanced  

  • 2011   Animal physiology advanced  

  • 2011   Animal physiology advanced  

  • 2011   Seminar of animal physiology  

  • 2011   Seminar of animal physiology  

  • 2011   Animal physiology advanced   Master's Course

  • 2011   Animal physiology advanced   Master's Course

▼display all

Research Interests

  • fish physiology

Published Papers

  • Acute toxicity of ammonia and nitrite to Siamese fighting fish (Betta splendens)

    Makiko Kajimura, Kazuyuki Takimoto, Ayaka Takimoto (Part: Lead author, Corresponding author )

    BMC Zoology ( Springer Science and Business Media LLC )  8 ( 1 )   2023.11

     View Summary

    Abstract

    The acute toxicity and sublethal effects of ammonia and nitrite on the air-beathing Siamese fighting fish, betta (Betta splendens) was studied for 96 h. The LC50 (50% Lethal Concentration) for 96 h for adult bettas to ammonia-N and nitrite-N was 123.4 mM (1.7 g/L, 95% confidence limits: 114.7–130.0 mM) and 24.6 mM (343.6 mg/L, 95% confidence limits: 22.7–26.4 mM) respectively. Exposure to 90 mM ammonia did not affect ammonia and urea excretion rates in bettas. There was no significant difference in values between control and ammonia-loaded (90 mM ammonia) individuals in either brain or liver activities of glutamine synthase, while plasma ammonia levels slightly increased. It appears unlikely that ammonia was converted to urea or amino acids for detoxification. Sublethal nitrite (24.6 mM nitrite) affected plasma nitrite, methemoglobin and hemoglobin. Plasma nitrite values remained much lower than ambient concentrations. Betta has a labyrinth organ and can breathe air. Bettas may temporarily reduce the entry of ammonia and nitrite into the body by increasing the rate of air respiration and reducing the contribution of the gill epithelium, which is highly permeable to these nitrogenous pollutants.

    DOI

  • 教職新科目「総合的な学習の時間の指導法」の実践 (1) : 授業内容と学生アンケートから

    谷尻 治, 貴志 年秀, 梶村 麻紀子, 古賀 庸憲

        2022.03  [Refereed]

    DOI

  • Environmental education using botanical name plates

    梶村 麻紀子, 中筋 千晶, 豊田 充崇, 教育学部, 宮井 健至, 高須 英樹 (Part: Lead author )

    和歌山大学教職大学院紀要 : 学校教育実践研究 ( [出版社不明] )  5   129 - 135   2021.03  [Refereed]

     View Summary

    和歌山大学教育学部附属特別支援学校の植栽調査を行い、その結果をもとに教材開発およびその実践を行った。植栽調査では校内に32種(栽培品種のミカン類を除く)の樹木および大型多年生草本があることを確認した。そして高等部の5名の生徒とともに樹名板を設置し、生徒には校内植物マップとそれぞれの植物についての解説文を作成してもらった。また、知識を定着させるために、ゲーム感覚で植物を学ぶビンゴゲームを考案し、生徒の作成した解説文を用いて学習するiPad 教材を開発し実践した。実践後の生徒の感想および、樹名板設置前後の植物名認知度アンケートの結果から、この実践は植物名を入り口として、植物そのものの形態や生態に興味を持ち、探求へのきっかけとなったことが示された。

    DOI

  • Observation of somatic cell division using green onion (Allium fistulosum L.).

    梶村麻紀子, 廣瀬正紀 (Part: Lead author )

    和歌山大学教育学部紀要 自然科学(Web)   71   2021

  • カニを用いた生き物観察の実践

    塩﨑祐斗、新明郁実、裏橋慶一、林和希、梶村麻紀子、古賀庸憲、廣瀬正紀、高須英樹

    和歌山大学教育学部紀要. 自然科学   68(1)   41-45   2018.01

  • 双眼実体顕微鏡を用いたクマムシ観察の授業実践

    梶村麻紀子・寺本由・廣瀬正紀・岩田 勝哉・古賀庸憲・荒木 良一・高須英樹

    和歌山大学教職大学院大学院紀要   2   179-184   2017  [Refereed]

  • 生活科を見据えた附属農場におけるトウモロコシ栽培を振り返って

    荒木良一・小林匡輔・梶村麻紀子・井嶋博

    和歌山大学教育学部紀要. 教育科学   68(1)   251-254   2017  [Refereed]

  • ヤドカリを用いた生物観察の実践

    和歌山大学教育学部教育実践総合センター紀要     2014.09  [Refereed]

  • 魚類の血漿タンパク質の定量実験について

    和歌山大学教育学部紀要. 教育科学     2014.02

  • Urea-based osmoregulation in the developing embryo of oviparous cartilaginous fish (Callorhinchus milii): contribution of the extraembryonic yolk sac during the early developmental period

    Wataru Takagi, Makiko Kajimura, Hironori Tanaka, Kumi Hasegawa, Justin Bell, Tes Toop, John Donald and Susumu Hyodo

    Journal of experimental biology     2013.12  [Refereed]

  • Hepatic and extrahepatic distribution of ornithine urea cycle enzymes in holocephalan elephant fish (Callorhinchus milii)

    Wataru Takagi, Makiko Kajimura, Justin D. Bell, Tes Toop, John A. Donald, Susumu Hyodo

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY ( ELSEVIER SCIENCE INC )  161 ( 4 ) 331 - 340   2012.04  [Refereed]

     View Summary

    Cartilaginous fish comprise two subclasses, the Holocephali (chimaeras) and Elasmobranchii (sharks, skates and rays). Little is known about osmoregulatory mechanisms in holocephalan fishes except that they conduct urea-based osmoregulation, as in elasmobranchs. In the present study, we examined the ornithine urea cycle (OUC) enzymes that play a role in urea biosynthesis in the holocephalan elephant fish, Callorhinchus milii (cm). We obtained a single mRNA encoding carbamoyl phosphate synthetase III (cmCPSIII) and ornithine transcarbamylase (cmOTC), and two mRNAs encoding glutamine synthetases (cmGSs) and two arginases (cmARGs), respectively. The two cmGSs were structurally and functionally separated into two types: brain/liver/kidney-type cinGS1 and muscle-type cmGS2. Furthermore, two alternatively spliced transcripts with different sizes were found for cmgs1 gene. The longer transcript has a putative mitochondrial targeting signal (MTS) and was predominantly expressed in the liver and kidney. MTS was not found in the short form of cmGS1 and cmGS2. A high mRNA expression and enzyme activities were found in the liver and muscle. Furthermore, in various tissues examined, mRNA levels of all the enzymes except cmCPSIII were significantly increased after hatching. The data show that the liver is the important organ for urea biosynthesis in elephant fish, but, extrahepatic tissues such as the kidney and muscle may also contribute to the urea production. In addition to the role of the extrahepatic tissues and nitrogen metabolism, the molecular and functional characteristics of multiple isoforms of GSs and ARGs are discussed. (C) 2011 Elsevier Inc. All rights reserved.

    DOI

  • The influence of feeding and fasting on plasma metabolites in the dogfish shark (Squalus acanthias)

    Chris M. Wood, Patrick J. Walsh, Makiko Kajimura, Grant B. McClelland, Shit F. Chew

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY ( ELSEVIER SCIENCE INC )  155 ( 4 ) 435 - 444   2010.04  [Refereed]

     View Summary

    Dogfish sharks are opportunistic predators, eating large meals at irregular intervals. Here we present a synthesis of data from several previous studies on responses in plasma metabolites after natural feeding and during prolonged fasting (up to 56 days), together with new data on changes in plasma concentrations of amino acids and non-esterified fatty acids. Post-prandial and long-term fasting responses were compared to control sharks fasted for 7 days, a typical inter-meal interval. A feeding frenzy was created in which dogfish were allowed to feed naturally on dead teleosts at two consumed ration levels, 2.6% and 5.5% of body weight. Most responses were more pronounced at the higher ration level. These included increases in urea and TMAO concentrations at 20 h, followed by stability through to 56 days of fasting. Ammonia levels were low and exhibited little short-term response to feeding, but declined to very low values during the extended fast. Glucose and beta-hydroxybutyrate both fell after feeding, the latter to a greater and more prolonged extent (up to 60 h), whereas acetoacetate did not change. During prolonged fasting, glucose concentrations were well regulated, but beta-hydroxybutyrate increased to 2-3-fold control levels. Total plasma amino acid concentrations increased in a biphasic fashion, with peaks at 6-20 h, and 48-60 h after the meal, followed by homeostasis during the extended fast. Essential and non-essential amino acids generally followed this same pattern, though some exhibited different trends after feeding: taurine, beta-alanine, and glycine (decreases or stability), alanine and glutamine (modest prolonged increases), and threonine, serine, asparagine, and valine (much larger short-term increases). Plasma non-esterified fatty acid concentrations declined markedly through 48 h after the 2.6% meal. These data are interpreted in light of companion studies showing elevations in aerobic metabolic rate, urea production, rectal gland function, metabolic base excretion, and activation of ornithine-urea cycle and aerobic enzymes after the meal, and muscle N-depletion but maintenance of osmolality and urea production during long-term fasting. (c) 2009 Elsevier Inc. All rights reserved.

    DOI

  • The influence of feeding and fasting on plasma metabolites in the dogfish shark (Squalus acanthias)

    Chris M. Wood, Patrick J. Walsh, Makiko Kajimura, Grant McClelland, Shit Fun Chew

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY ( ELSEVIER SCIENCE INC )  153A ( 2 ) S66 - S67   2009.06

    DOI

  • Enzymatic and mitochondrial responses to 5 months of aerial exposure in the slender lungfish Protopterus dolloi

    J. F. Staples, M. Kajimura, C. M. Wood, M. Patel, Y. K. Ip, G. B. McClelland

    JOURNAL OF FISH BIOLOGY ( WILEY-BLACKWELL )  73 ( 3 ) 608 - 622   2008.08  [Refereed]

     View Summary

    Mitochondrial respiration and activities of key metabolic enzymes from liver and white skeletal muscle were compared between control aquatic slender lungfish Protopterus dolloi, and those exposed to air for 5 months. Activities of citrate synthase, glycogen phosphorylase, phosphofructokinase and pyruvate kinase in liver were not affected by air-exposure. In muscle, air-exposure reduced citrate synthase and pyruvate kinase activities (relative to tissue wet mass) by 63 and 50%, respectively. Liver carnitine palmitoyl transferase activity (relative to mitochondrial protein) decreased by half following air-exposure, but there was no change in muscle. In mitochondria isolated from muscle, state 3 and state 4 respiration were reduced by 74 and 89%, respectively following air-exposure, but liver mitochondria were not affected. In liver, air-exposure increased activities of ornithine-urea cycle enzymes including glutamine synthase, carbamoyl-phosphate synthase III and arginase, by 1.9- to 4.2-fold. Carbamoyl-phosphate synthase III activity could not be detected in muscle, indicating that urea is not synthesized in this tissue. These data suggest that skeletal muscle metabolism is downregulated in air-exposure, conserving energy and protein during a period when the animals cannot forage. In contrast, ATP production capacities in the liver are maintained, and this may permit expensive urea biosynthesis to continue during aerial exposure. (C) 2008 The Authors Journal compilation (C) 2008 The Fisheries Society of the British Isles.

    DOI

  • Intertissue regulation of carnitine palmitoyltransferase I (CPTI): Mitochondrial membrane properties and gene expression in rainbow trout (Oncorhynchus mykiss)

    Andrea J. Morash, Makiko Kajimura, Grant B. McClelland

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES ( ELSEVIER SCIENCE BV )  1778 ( 6 ) 1382 - 1389   2008.06  [Refereed]

     View Summary

    Carnitine palmitoyltransferase (CPT) I is regulated by several genetic and non-genetic factors including allosteric inhibition, mitochondrial membrane composition and/or fluidity and transcriptional regulation of enzyme content. To determine the intrinsic differences in these regulating factors that may result in differences between tissues in fatty acid oxidation ability, mitochondria were isolated from red, white and heart muscles and liver tissue from rainbow trout. Maximal activity (V-max) for beta-oxidation enzymes and citrate synthase per mg tissue protein as well as CPT I in isolated mitochondria followed a pattern across tissues of red muscle> heart> white muscle liver suggesting both quantitative and qualitative differences in mitochondria. CPT I inhibition showed a similar pattern with the highest malonyl-CoA concentration to inhibit activity by 50% (IC50) found in red muscle while liver had the lowest. Tissue malonyl-CoA content was highest in white muscle with no differences between the other tissues. Interestingly, the gene expression profiles did not follow the same pattern as the tissue enzyme activity. CPT I mRNA expression was greatest in heart> red muscle>white muscle>liver. In contrast, PPAR alpha mRNA was greatest in the liver>red muscle> heart> white muscle. There were no significant differences in the mRNA expression of PPAR between tissues. As well, no significant differences were found in the mitochondrial membrane composition between tissues, however, there was a tendency for red muscle to exhibit higher proportions of PUFAs as well as a decreased PC:PE ratio, both of which would indicate increased membrane fluidity. In fact, there were significant correlations between IC50 of CPT I for malonyl-CoA and indicators of membrane fluidity across tissues. This supports the notion that sensitivity of CPT I to its allosteric regulator could be modulated by changes in mitochondrial membrane composition and/or fluidity. (C) 2008 Elsevier B.V. All rights reserved.

    DOI

  • Is the alkaline tide a signal to activate metabolic or ionoregulatory enzymes in the dogfish shark (Squalus acanthias)?

    Chris M. Wood, Makiko Kajimura, Thomas P. Mommsen, Patrick J. Walsh

    PHYSIOLOGICAL AND BIOCHEMICAL ZOOLOGY ( UNIV CHICAGO PRESS )  81 ( 3 ) 278 - 287   2008.05  [Refereed]

     View Summary

    Experimental metabolic alkalosis is known to stimulate whole-animal urea production and active ion secretion by the rectal gland in the dogfish shark. Furthermore, recent evidence indicates that a marked alkaline tide (systemic metabolic alkalosis) follows feeding in this species and that the activities of the enzymes of the ornithine-urea cycle (OUC) for urea synthesis in skeletal muscle and liver and of energy metabolism and ion transport in the rectal gland are increased at this time. We therefore evaluated whether alkalosis and/or NaCl/volume loading (which also occurs with feeding) could serve as a signal for activation of these enzymes independent of nutrient loading. Fasted dogfish were infused for 20 h with either 500 mmol L(-1) NaHCO(3) (alkalosis + volume expansion) or 500 mmol L(-1) NaCl (volume expansion alone), both isosmotic to dogfish plasma, at a rate of 3 mL kg(-1) h(-1). NaHCO3 infusion progressively raised arterial pH to 8.28 (control = 7.85) and plasma [HCO(3)(-)] to 20.8 mmol L(-1) (control = 4.5 mmol L(-1)) at 20 h, with unchanged arterial Pco(2), whereas NaCl/volume loading had no effect on blood acid-base status. Rectal gland Na(+),K(+)-ATPase activity was increased 50% by NaCl loading and more than 100% by NaHCO(3) loading, indicating stimulatory effects of both volume expansion and alkalosis. Rectal gland lactate dehydrogenase activity was elevated 25% by both treatments, indicating volume expansion effects only, whereas neither treatment increased the activities of the aerobic enzymes citrate synthase, NADP-isocitrate dehydrogenase, or the ketone body-utilizing enzyme beta-hydroxybutyrate dehydrogenase in the rectal gland or liver. The activity of ornithine-citrulline transcarbamoylase in skeletal muscle was doubled by NaHCO(3) infusion, but neither treatment altered the activities of other OUC-related enzymes (glutamine synthetase, carbamoylphosphate synthetase III). We conclude that both the alkaline tide and salt loading/volume expansion act as signals to activate some but not all of the elevated metabolic pathways and iono-regulatory mechanisms needed during processing of a meal.

    DOI

  • Natural feeding influences protein expression in the dogfish shark rectal gland: A proteomic analysis

    W. Wesley Dowd, Chris M. Wood, Makiko Kajimura, Patrick J. Walsh, Dietmar Kultz

    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS ( ELSEVIER SCIENCE INC )  3 ( 1 ) 118 - 127   2008.03  [Refereed]

     View Summary

    The rectal gland is the principal salt-secreting organ in elasmobranchs, yet its functional response to normal physiological variation (e.g., due to feeding, stress) has only recently been examined. To complement studies on acid-base, digestive, and osmoregulatory physiology in response to natural feeding, we investigated protein-level responses in the rectal gland of spiny dogfish (Squalus acanthias) 6 h, 20 h, and 5 days (reference control) after a meal. Our objective was to identify proteins involved in regulation of osmoregulatory and metabolic processes in response to feeding. Proteins were separated by two-dimensional gel electrophoresis, and protein spots that were significantly up- or down-regulated >2 fold (i.e., abundance increased more than 100% or decreased more than 50%) were detected using gel image analysis software. Of 684 proteins analyzed on 2D gels, 16 proteins changed significantly 6 h after feeding vs. 5 day controls (5 decreased; 11 increased), and 12 proteins changed > 2 fold 20 h after feeding vs. 5 day controls (2 decreased; 10 increased). Thirteen of these proteins were identified using mass spectrometry and classified into functional pathways using the PANTHER bioinformatics database. Rectal gland proteins that were regulated following feeding fell into three main categories: cytoskeletal/muscular (e.g., tropomyosin alpha chain, transgelin), energy metabolism (e.g., malate dehydrogenase, ATP synthase), and nucleotide metabolism (nucleoside diphosphate kinase). The data also revealed that previously documented increases in the activity of isocitrate dehydrogenase after feeding are at least partially due to increased abundance of a cytosolic, NADP-dependent isoform of this enzyme. One of the primary components of the rectal gland's response to feeding appears to be maintenance of the cellular supply of energy, which would be necessary to fuel increased activities of enzymes involved in salt secretion and oxidative metabolism in the rectal gland following a meal. (C) 2007 Elsevier Inc. All rights reserved.

    DOI

  • The spiny dogfish Squalus acanthias L. maintains osmolyte balance during long-term starvation

    M. Kajimura, P. J. Walsh, C. M. Wood

    JOURNAL OF FISH BIOLOGY ( BLACKWELL PUBLISHING )  72 ( 3 ) 656 - 670   2008.02  [Refereed]

     View Summary

    Over 56 days of starvation, spiny dogfish Squalus acanthias lost c. 0.3% body mass per day and condition factor and hepato-somatic index declined, but plasma osmolality levels remained higher than seawater levels for the entire period; major osmolytes in the plasma, such as urea, trimethylamine oxide and inorganic ions did not change appreciably. Urea was always the dominant nitrogen waste and was excreted at a constant rate over the 56 day starvation period, suggesting that a minimum rate of urea loss to the environment is unavoidable. Significant amounts of unknown-nitrogen compounds were also excreted at rates higher than that of ammonia. The dogfish can maintain its osmolytes constant despite losing large amounts of nitrogen-rich urea, and therefore maintains plasma hyperosmotic regulation over long-term starvation. (c) 2008 The Authors Journal compilation (c) 2008 The Fisheries Society of the British Isles.

    DOI

  • Osmoregulation, ionoregulation and acid-base regulation by the gastrointestinal tract after feeding

    Journal of Experimental Biology. The Company of Biologists (イギリス) 第210巻: 8号1335~1349頁     2007.04  [Refereed]

  • Rapid Regulation of Na+ fluxes and Ammonia Excretion in Response to Acute Environmental Hypoxia in t

    American Journal of Physiology Regulatory-Integrative and Comparative Physiology. The American Physiological Society(アメリカ) 第292巻5号R2048~R2058頁.     2007.02  [Refereed]

  • The African lungfish (Protopterus dolloi): Osmoregulation and ionoregulation in a fish out of water.

    WILKIE MP

    Physiological and Biochemical Zoology. Chicago Journals(アメリカ) 第80巻1号99-112頁   80   99 - 112   2007.01  [Refereed]

  • Metabolic organization and effects of feeding on enzyme activities of the dogfish shark (Squalus aca

    Journal of Experimental Biology. The Company of Biologists (イギリス) 第209巻15号2929~2938頁     2006.08  [Refereed]

  • The dogfish shark (Squalus acanthias) increases both hepatic and extra-hepatic ornithine- urea cycle

    Physiological and Biochemical Zoology. Chicago Journals (アメリカ) 第79:巻3号 602~613頁     2006.05  [Refereed]

  • Tribute to R.G.Boutilier: The effect of size on the physiological and behavioural responses of oscar

    Journal of Experimental Biology. The Company of Biologists (イギリス) 第209巻7号1197~1205頁     2006.04  [Refereed]

  • High ambient ammonia promotes growth in an ureogenic goby, Mugilogobius abei.

    IWATA K.

    Journal of Comparative Physiology Part B. Springer (ドイツ) 第175巻6号395~404頁   175   395 - 404   2005.07  [Refereed]

    DOI

  • Alkaline tide and nitrogen conservation after feeding in an elasmobranch (Squalus acanthias).

    Journal of Experimental Biology. The Company of Biologists(イギリス) 第208巻14号2693~2705頁     2005.07  [Refereed]

  • Dogmas and controversies in the handling of nitrogenous wastes: The effect of feeding and fasting on

    Journal of Experimental Biology. The Company of Biologists (イギリス) 第207巻12号1993~2002頁     2004.05  [Refereed]

  • Diurnal nitrogen excretion rhythm of the functionally ureogenic gobiid fish Mugilogobius abei.

    Comparative Biochemistry and Physiology Part B. Elsevier (オランダ) 第131巻2号227~239頁     2002.02  [Refereed]

  • Evolution of a ureagenic ability of Japanese Mugilogobius species (Pisces: Gobiidae)

    Takahiko Mukai, Makiko Kajimura, Katsuya Iwata

    Zoological Science ( Zoological Society of Japan )  17 ( 4 ) 549 - 557   2000  [Refereed]

     View Summary

    Interspecific differences in the ability to excrete urea in four gobiid fishes, Mugilogobius abei, M. chulae, M. sp. 1 and M. sp. 2, in response to elevated external ammonia, were compared and set against the phylogenetic relationships of species, following molecular phylogenetic analyses using a portion of mitochondrially-encoded 12S ribosomal RNA and tRNA-Val genes, which were sequenced from five species of Mugilogobius. The resulting tree, based on 710 base pairs of aligned sequences, was statistically robust and indicated two major clades, (M. abei, M. sp. 1 and M. chulae) and (M. parvus and M. sp. 2), respectively. High ureagenic ability was demonstrated in M. abei and M. sp. 1, being sister species according to the phylogenetic analysis, while the remaining species having a low level of ability. The two former species are restricted to temperate and subtropical Japan, whereas the others are widely distributed throughout tropical Asia. The most parsimonious reconstruction of these features suggested that ancestral Mugilogobius, probably widely distributed in tropical Asia, may had low ureagenic ability. The high ureagenic ability of the temperate species may have evolved concurrently with the northward spread of Mugilogobius from the tropical zone.

    DOI

  • Functional ureogenesis in the gobiid fish, Mugilogobius abei

    K. Iwata, M. Kajimura, T. Sakamoto

    Journal of Experimental Biology   203 ( 24 ) 3703 - 3715   2000  [Refereed]

     View Summary

    To examine the transition to ureogenesis, the gobiid fish Mugilogobius abei was immersed in 2 mmoll-1 NH4HCO3 or a 15N-labelled ammonia solution [1 mmoll-1 (15NH4)2SO4, pH 8.0] for 4-8 days. When exposed to 2 mmoll-1 NH4HCO3 or 15N-labelled ammonia solution for 4 days, the rate of urea excretion increased to seven times that of the control (in 20 % synthetic sea water) and remained at this level for 4 days. The proportion of nitrogen excreted as urea reached 62 % of total nitrogen excretion (ammonia-N + urea-N). 15N-enrichment of the amide-N in glutamine in the tissues of fish exposed to 15N-labelled ammonia was virtually the same as that of ammonia-N: i.e. approximately twice that of urea-N in the excreta and the tissues. Glutamine contents and glutamine synthetase activities in the liver and muscle increased greatly following exposure to ammonia. Urea and citrulline contents in the muscle and whole body of the exposed fish increased significantly, whereas uric acid contents remained unchanged. Carbamoyl phosphate synthetase III (CPSase III) mRNA expression and CPSase III activity were detected in the muscle, skin and gill, but levels were negligible in the liver. Furthermore, all other ornithine-urea cycle (O-UC) enzymes were also detected in muscle, skin and gill. Thus, M. abei clearly shows the transition from ammoniotely to ureotely under ammonia-loading condition and is able to produce urea mainly via the O-UC operating in multiple non-hepatic tissues as a means for ammonia detoxification.

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Books etc

  • 魚類学の百科事典

    日本魚類学会( Part: Contributor)

    丸善出版  2018.10  ISBN: 9784621303177

  • 動物生理学[原書第5版] 環境への適応 第8章 水と浸透圧,第9章 排出

    2007.09 

Misc

  • 創作的活動を取り入れた教科横断型プログラミング教育の実践—A Trial of Cross-Curricular Programming Education Incorporating Creative Activities.

    梶村 麻紀子, 豊田 充崇, 望月 純子

    和歌山大学教育学部紀要. 教育科学 ( 和歌山大学教育学部 )  73   83 - 90   2023.02

     View Summary

    As a new trial of elementary school programming education, we took pictures of painted fish on paper with a tablet device and programmed the fish to move as desired. The goal of this practice was to improve “observation and recording” skills, which is a subject objective of Life Science and Science; to develop expressive skills, which is an objective of Arts and Crafts; and to foster computational thinking, which is an objective of programming education. The results of the trial lesson proved that it was developmentally appropriate for the target students, and the studentsʼ motivation and awareness of programming were improved, indicating that the lesson could be positioned as a cross-curricular class.

    DOI

Conference Activities & Talks

  • 和歌山大学地内と海南市不動谷の里山で見られる鳥類とその繁殖行動について

    武井恒興, 有本智, 高須英樹, 梶村麻紀子

    南紀生物同好会研究発表大会  2023.12.10  

  • 大阪府、男里川河口域における魚類群集の周年変化

    土田和幸, 平嶋健太郎, 梶村麻紀子

    南紀生物同好会研究発表大会  2023.12.10  

  • 大阪湾南部におけるクサフグの成熟

    井上帆琉, 平嶋健太郎, 梶村麻紀子

    南紀生物同好会研究発表大会  2023.12.10  

  • Hypoxia tolerance of dragonfly larvae in various habitats

    YOSHIMURA Toshitaka, MATSUNO Shigetomi, KAJIMURA Makiko

    The 45th Annual Meeting of JSCPB2023 in Osaka  2023.12.02  

  • 加太・潮岬におけるタイドプールの魚類群集の比較

    南紀生物同好会研究発表大会  2022.12  

  • 紀ノ川および貴志川本流におけるトンボ目の種構成について

    南紀生物同好会研究発表大会  2022.12  

  • Acute toxicity of ammonia and nitrite to betta fish

    JSCPB2022  2022.11  

  • 血液の教材化 ― 魚類を材料にして ―

    梶村麻紀子

    日本理科教育学会近畿支部大会  2013.11   日本理科教育学会近畿支部大会実行委員会

  • 体験を通して学ぶ生物多様性

    加藤健司, 矢野充博, 梶村麻紀子

    日本理科教育学会近畿支部大会  2013.11   日本理科教育学会近畿支部大会実行委員会

  • 卵生軟骨魚類の発生初期における体液調節:卵黄嚢上皮から胚体への機能移行

    高木亙,田中宏典,梶村麻紀子,ドナルドジョン,トゥープテス,兵藤晋

    日本動物学会第83回  2012.09   日本動物学会

  • 卵生軟骨魚類の発生過程における浸透圧調節

    高木 亙・田中宏典・Donald John・Toop Tes・梶村麻紀子・兵藤 晋

    日本水産学会春季大会  2012.03  

  • Ornithine urea cycle enzymes in holocephalan elephant fish (Callorhinchus milii)

    Takagi W, Kajimura M, Bell JD, Toop T, Donald JA, Hyodo S.

    the 8th International Congress on Comparative Physiology and Biochemistry  2011   比較生理生化学会

  • 摂餌が海産板鰓類の尿素合成におよぼす影響

    梶村麻紀子、Walsh J. Patrick、Mommsen P. Thomas、Wood M. Chris

    第34回日本比較内分泌学会大会日本比較生理生化学会第31回大会 合同大会CompBiol2009  2009   日本比較内分泌学会大会日本比較生理生化学会

  • Changes in protein expression in the dogfish shark rectal gland following natural feeding.

    Annual Meeting of the Society for Integrative and Comparative Biology.  2008   Society for Integrative and Comparative Biology

  • Natural feeding influences protein expression in the dogfish rectal gland. Poster presentation

    American Fisheries Society  2007   American Fisheries Society 137th Annual Meeting

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Instructor for open lecture, peer review for academic journal, media appearances, etc.

  • 認定講習講師

    2023.08.26
    -
    2023.08.27

    和歌山県教育委員会

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    非常勤講師

    令和5年度和歌山県教育委員会教育職員免許法認定講習の講師

  • 自然観察教室

    2023.01.24

    その他

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    出張講座

    海南市立五月山保育園

  • 自然観察教室

    2022.12.06

    その他

     View Details

    出張講座

    海南市立五月山保育園

  • 育児講座

    2022.10.19

    その他

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    出張講座

    海南市地域子育て支援センターさつきやま

  • 自然観察教室

    2022.09.13

    その他

     View Details

    出張講座

    海南市立五月山保育園

  • 自然観察教室-観察する目を養う③

    2022.01.26

    その他

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    出張講座

    プログラミング教育、海南市立五月山保育園,日付:26日

  • 海南市子育て支援センター・育児講座

    2021.11.17

    その他

     View Details

    出張講座

    ボトルアクアリウムで生き物観察、海南市立五月山保育園,日付:17日

  • 教科横断型プログラミング教育

    2021.11.09

    岬町立深日小学校

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    プログラミング、理科

    プログラミングアプリの一つであるプログラミングゼミを使って、児童が描いた海の生き物を画面上で動かすプログラミング指導を行った。

  • 自然観察教室-観察する目を養う②

    2021.09.22

    その他

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    出張講座

    セミの抜け殻観察、海南市立五月山保育園,日付:22日

  • 講師

    2021.09.04
    -
    2021.09.05

    和歌山県教育委員会

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    講演講師

    和歌山県教育委員会教育職員免許法認定講習(理科)に係る講義

  • 自然観察教室-観察する目を養う①

    2021.06.30

    その他

     View Details

    出張講座

    種と実の観察、海南市立五月山保育園,日付:30日

  • 教科横断型プログラミング教育

    2021.06.28

    和歌山市立 中之島小学校

     View Details

    プログラミング、理科

    プログラミングアプリの一つであるscratchを使って、児童が描いた海の生き物を画面上で動かすプログラミング指導を行った。

  • 自然観察教室-観察する目を養う③

    2021.02.24

    その他

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    出張講座

    プログラミング、海南市立五月山保育園,日付:4日

  • 植物(学校植栽)の解説

    2020.12.07

    和歌山大学教育学部附属小学校

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    講演講師等

    講師

  • 自然観察教室-観察する目を養う②

    2020.09.04

    その他

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    出張講座

    昆虫の観察、海南市立五月山保育園,日付:4日

  • 自然観察教室-観察する目を養う①

    2020.06.26

    その他

     View Details

    出張講座

    種と実の観察、海南市立五月山保育園,日付:26日

  • 講師

    2019.12

    和歌山県立海南高等学校SSH第2学年冬季特設課外授業

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    講演講師等

    講師,任期:2019年12月~

  • まちかど土曜楽交

    2016.04

    その他

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    小・中・高校生を対象とした学部体験入学・出張講座等

    まちかど土曜楽交

  • 加太磯観察会

    2013.07

    その他

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    小・中・高校生を対象とした学部体験入学・出張講座等

    和歌山大学附属中学校

  • スーパー・サイエンティスト・ジュニア(SSJ)

    2013.04

    和歌山大学学生自主創造科学センター,おもしろ科学まつり実行委員会

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    公開講座・講演会の企画・講師等

    小学生がサポートの大学生と一緒に,半年かけて一つのサイエンスプロジェクトを行い,おもしろ科学まつりで発表する

  • SSH 冬季特設課外授業

    2013.04

    その他

     View Details

    小・中・高校生を対象とした学部体験入学・出張講座等

    海南高校

  • 知の冒険旅行

    2013.04

    その他

     View Details

    小・中・高校生を対象とした学部体験入学・出張講座等

    和歌山大学附属中学校

  • おもしろ科学まつり

    2012.04

    青少年のための科学の祭典・和歌山大学教育学部など

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    公開講座・講演会の企画・講師等

    県内のこどもたちに科学を体験させる

  • スーパーサイエンスハイスクール授業および国際交流授業

    2012.04

    その他

     View Details

    小・中・高校生を対象とした学部体験入学・出張講座等

    向陽高校,Dartford Grammar School ,日付:29 10 2012

  • SSH 冬季特設課外授業

    2012.04

    その他

     View Details

    小・中・高校生を対象とした学部体験入学・出張講座等

    海南高校,日付:11 12 2012

  • 知の冒険旅行

    2012.04

    その他

     View Details

    小・中・高校生を対象とした学部体験入学・出張講座等

    和歌山大学附属中学校,日付:9 21 2012

  • おもしろ科学まつり

    2011.04

    青少年のための科学の祭典・和歌山大学教育学部など

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    公開講座・講演会の企画・講師等

    県内のこどもたちに科学を体験させる

  • 特設課外授業

    2011.04

    その他

     View Details

    小・中・高校生を対象とした学部体験入学・出張講座等

    海南高校,日付:6 12 2011

  • スーパーサイエンスハイスクール授業

    2011.04

    その他

     View Details

    小・中・高校生を対象とした学部体験入学・出張講座等

    向陽高校,Dartford Grammar School ,日付:18 10 2011

  • 知の冒険旅行

    2010.04

    その他

     View Details

    小・中・高校生を対象とした学部体験入学・出張講座等

    和歌山大学附属中学校,日付:6 17 2010

  • おもしろ科学まつり

    2010.04

    青少年のための科学の祭典・和歌山大学教育学部など

     View Details

    公開講座・講演会の企画・講師等

    県内のこどもたちに科学を体験させる

  • 特設課外授業

    2010.04

    その他

     View Details

    小・中・高校生を対象とした学部体験入学・出張講座等

    海南高校,日付:9 12 2010

  • サイエンス・パートナーシップ・プログラム

    2009.04

    和歌山県教育委員会

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    公開講座・講演会の企画・講師等

    観察・実験による苦手科目の力量向上

  • おもしろ科学まつり

    2009.04

    青少年のための科学の祭典・和歌山大学教育学部など

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    公開講座・講演会の企画・講師等

    県内のこどもたちに科学を体験させる

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Course for renewal of teaching license, teacher-librarian course, etc. (contracted business)

  • 2021  【選択】生物の観察と実験(教員免許状更新講習)

  • 2021  免許法認定講習

  • 2020  【選択】生物の観察と実験(コロナ対策のため開講中止)(教員免許更新講習)